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Preliminary Analysis of the Expression Pattern of Soybean acyl-ACP-Thioesterase Gene and Promoter(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年05期
Page:
714-717
Research Field:
Publishing date:

Info

Title:
Preliminary Analysis of the Expression Pattern of Soybean acyl-ACP-Thioesterase Gene and Promoter
Author(s):
ZHAO YanQI Hong-yingFAN Zhen-yuYANG Xiao-jie
College of Life Science and Agro-forestry,Qiqihaer University,Qiqihaer 161006,Heilongjiang,China
Keywords:
Soybeanacyl-ACP thioesterasePromoterCloning
PACS:
Q756
DOI:
10.3969/j.issn.1000-9841.2012.05.005
Abstract:
The expression pattern of soybean acyl-ACP thioesterase gene in soybean tissues was detected by real-time quantitative PCR(RTQ-PCR).The result showed that there were a little activity in roots,stems,leaves and flowers,but there was higher activity in seeds.The 5′-flanking upstream sequence of soybean acyl-ACP thioesterase gene,named AP,was isolated from soybean genomic DNA by PCR method,and the length was 2 057 bp.Sequence analysis by PLACE revealed that this fragment contained a series of motifs related to seed-specific promoters,such as RY repeat,SEF1 motif,SEF3 motif,SEF4 motif,E-box and ACGT.It can be inferred that AP promoter possess the function driven downstream gene expression exclusively in soybean seeds.

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Last Update: 2014-08-18