|Table of Contents|

Effect of Xylo-oligosaccharide on Growth and Resistance to SMV in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年04期
Page:
621-624,629
Research Field:
Publishing date:

Info

Title:
Effect of Xylo-oligosaccharide on Growth and Resistance to SMV in Soybean
Author(s):
ZHANG Xiu-xiu; WU Hui-jun; ZHOU Yu-cheng; GAO Xue-wen
College of Plant Protection/Key Laboratory of Integrated Management of Crop Diseases and Pests,Ministry of Education,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China
Keywords:
Xylo-oligosaccharide; Soybean; Growth promotion; SMV resistance; Quantitative RT-PCR
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.04.022
Abstract:
Treated soybean with different concentrations of xylo-oligosaccharide to study the effect of xylo-oligosaccharide on growth and resistance to soybean mosaic virus(SMV)in soybean.The results of greenhouse experiment showed that 18 days after the seed of soybean were dealt with the xylo-oligosaccharide solution,compared with the contol, 50 mg·L-1 xylo-oligosaccharide obviously increased the height,fresh weight,and main root length of soybean seedlings.Inoculated with SMV after 18 hours of pre-atomized the soybean leaves with xylo-oligosaccharide,compared with the contol,the disease index of soybean mosaic virus was obviously reduced.Using Quantitative RT-PCR to test the expression of the defense-related gene of the soybean leaves dealt with xylo-oligosaccharide,compared with the contol,the defense-related gene PR2,PR10,PR12 and LOX2had the highest related expression quantity at the time of 12 hours, PR3 at 18 hours,but PR1,PAL,PPO,and CHS at 24 hours.Results suggest that xylo-oligosaccharide can active salicylic acid(SA)and jasmonic acid(JA)signaling pathways of soybean,and enhances its resistance against SMV.

References:

[1]陈利锋,徐敬友.农业植物病理学[M].北京:中国农业出版社,2001:220-222.(Chen L F,Xu J Y.Agricultural phytopathology[M].Beijing:Agricultural Press,2001:220-222.)

[2]姬小明,吕全建,赵铭钦.低聚木糖的特性及应用研究进展[J].安徽农业科学,2007,35(23):7088-7089.(Ji X M,LüQ J,Zhao M Q.Research progress of characteristics and application of xylo-oligosaccharide[J].Journal of Anhui Agricultural Sciences,2007,35(23):7088-7089.)

[3]贺英.几种低聚糖激发子的制备及其对毛白杨愈伤组织抗病性诱导作用的研究[D].杨凌:西北农林科技大学,2006:30-34.(He Y.Preparation of various oligosaccharides elicitors and research on disease resistance of Populus tomentosa?callus induced by different ellcitors[D].Yangling:Northwest Agriculture and Forestry University,2006:30-34.)

[4]Yamaguchi T,Ito Y,Shibuya N.Oligosaccharide elicitors and their receptors for plant defense responses[J].Trends in Glycoscience and Glycotechnology,2000,12(64):113-120.

[5]Zhi H J,Gai J Y.Performances and germplasm evaluation of quantitative resistance to soybean mosaic virus in soybeans[J].Agricultural Science in China,2004,3(4):247-253.

[6]Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J].Methods,2001,25(4):402-408.

[7]Aguilar F,Montandon P E,Stutz E.Two genes encoding the soybean translation elongation factor eEF-1 alpha are transcribed in seedling leaves[J].Plant Molecular Biology,1991,17:351-360.

[8]Kim C S,Yi S Y,Lee Y K,et al.Isolation and differential expression of an acidic PR-1 cDNA gene from soybean hypocotyls infected with Phytophthora sojae?f.sp.glycines[J].The Plant Pathology Journal,2000,16:9-18.

[9]Takeuchi Y,Yoshikawa M,Takeba G,et al.Molecular cloning and ethylene induction of mRNA encoding a phytoalexin elicitor-releasing factor,β-1,3-endoglucanase,in soybean[J].Plant Physiology,1990,93:673-682.

[10]Gijzen M,Kuflu K,Qutob D,et al.A class I chitinase from soybean seed coat[J].Journal of Experimental Botany,2001,52:2283-2289.

[11]Ludwig A,Tenhaken R.Defence gene expression in soybean is linked to the status of the cell death program[J].Plant Molecular Biology,2000,44:209-218.

[12]Thomma B P H J,Cammue B P A,Thevissen K.Plant defensins[J].Planta,2002,216:193-202.

[13]Frank R L,Vodkin L O.Sequence and structure of a phenylalanine ammonia-lyase gene from Glycine max[J].Mitochondrial DNA,1991,1(5):335-346.

[14]Robert G U,Martha E R.Defense-related gene expression in soybean leaves and seeds inoculated with Cercospora kikuchii?and Diaporthe phaseolorum?var.meridionalis[J].Physiological and Molecular Plant Pathology,2010,75:64-70.

Memo

Memo:
-
Last Update: 2014-08-18