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Lithium Chloride,an Alternative Selectable Marker for Soybean Transformation(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年04期
Page:
552-556,567
Research Field:
Publishing date:

Info

Title:
Lithium Chloride,an Alternative Selectable Marker for Soybean Transformation
Author(s):
HUANG Wen-jia; LI Yong-guang; WANG Zhi-kun; LI Wei-na; WANG Tao; WANG Peng-peng; LI Wen-bin
Soybean Research Institute,Northeast Agricultural University/Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Biology and Genetics & Breeding for Soybean in Northeast China,Ministry of Agriculture，Harbin 150030,Heilongjiang,China
Keywords:
Soybean; Transgenic plant; Lithium Chloride; GUS dying; PCR
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.04.009
Abstract:
The proper concentration of lithium chloride and selection time were determined by the yellowing rate and the height of cluster buds of Dongnong50,treated with different concentration of lithium chloride.The data showed the suitable selective concentration of lithium choride and treated time were 60 mmol·L-1 and 10 day,respectively.Further analysis revealed that 80% of cluster buds resistant to lithium chloride was positive after GUS dying.The transgenic plants were detected by PCR analysis.The result suggested the optimized concentration of lithium chloride was 700 mmol·L-1and the lineages of the transgenic plants could normally grow and develop.Results suggested that the selected marker of lithium chloride could be applied in rapid selection for transgenic soybean plants.

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Last Update: 2014-08-18