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Cloning and Expression Analysis of Usp1 Gene from Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年04期
Page:
546-551
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Usp1 Gene from Soybean
Author(s):
HUANG ShanWANG Wei-qiHOU Wen-sheng
MOA Key Laboratory of Soybean Biology,National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China
Keywords:
SoybeanUspASequence analysisGene expression
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.04.008
Abstract:
According to the EST sequences of the Suppression Subtractive Hybridization(SSH)cDNA libraries,a soybean UspA gene named by GmUspl was cloned from the soybean by RT-PCR.The complete cDNA of GmUsp1 was 1 047 bp,it encoded a peptide which contained 164 amino acid residues.The 495 bp CDS showed that it belonged to the universal stress protein(UspA)family,by using the BLASTp tool of NCBI database.Homology analysis showed GmUsp1 had the highest similarity with the bean Vf_enod18,and it belonged to the group of UspA,which represented by the ATP-binging structure of the MJ-0577 protein from Methanococcus jannaschii.It also had the similar protein secondary structure with MJ-0577.In the salt sensitive variety Union and tolerant variety Wenfeng No.7,the expression of GmUsp1 was induced by 250 mmol·L-1 NaCl,100 μmol·L-1 ABA and 30% PEG-600 treatment,however,it had different response time and expression level.So GmUsp1 might be involved in plant stress adaptation,it suggested that GmUsp1 may play a role in salt stress adaptation in soybean.

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Last Update: 2014-08-18