Feasibility Analysis of Using Glyphosate as the Selective Agent in Soybean Genetic Transformation System with Cotyledonary Nodes as Explants(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年04期

Page:

538-541

Research Field:

Publishing date:

Info

Title:

Feasibility Analysis of Using Glyphosate as the Selective Agent in Soybean Genetic Transformation System with Cotyledonary Nodes as Explants

Author(s):

YUE Yan-lei¹; 2; 3; YU Li-jie¹; SUN Shi²; HAN Tian-fu²; HOU Wen-sheng²

1.Life Science and Technology College,Harbin Normal University,Harbin 150025,Heilongjiang;

2.MOA Key Lab of Soybean Biology(Beijing),National Key Facility for Crop Resources and Genetic Improvement,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081;

3.Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences,Mudanjiang 157041,Heilongjiang,China

Keywords:

Soybean transformation; Glyphosate; Selective agent

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.04.006

Abstract:

Using four conventional soybean varieties(Zigongdongdou,Jilinxiaoli-1,Zhonghuang 30 and Williams 82)and two glyphosate-resistant transgenic soybean lines(Zhongzuo J9331 and J9333),the optimal concentrations of glyphosate were explored at three stages of soybean genetic transformation system with the cotyledon nodes as explants.The results showed that it is feasible to use glyphosate as the selective agent,the suitable concentrations are 100-160,220-240 and 5-10 mg·L^-1 respectively at the stages of the induction, sereening and elongation of multiple shoots clumps. In the whole,the optimal screening effect can be gotten with the glyphosate concentrotions as 60,240 and 5 mg·L^-1 respectively at the three stages.

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