|Table of Contents|

Rapid Detecting Glufosinate-Tolerant Transgenic Soybean via Seed Germination Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年04期
Page:
534-537
Research Field:
Publishing date:

Info

Title:
Rapid Detecting Glufosinate-Tolerant Transgenic Soybean via Seed Germination Method
Author(s):
CHANG JingMENG Fan-liLI Yong-guangLI Wen-bin
Soybean Research Institute,Northeast Agricultural University,Chinese Education Ministry’s Key Laboratory of Soybean Biology,Agricultural Ministry’s Northeast Areal Key Laboratory of Soybean Biology and Genetic Breeding,Harbin 150030,Heilongjiang,China
Keywords:
Transgenic soybeanGlufosinate toleranceGermination methodMolecular assay
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.04.005
Abstract:
We need to choose a repaid and effective method to solve the problem that transgenic offsprings are genetic instable.In this study,the seeds of Dongnong 50 were sowed in MS medium contained different concentrations of glufosinate.Under 5.0 mg·L-1 glufosinate,the germination rate and growth of non-transgenic recipient Dongnong 50 were seriously inhibited and couldn’t complete life cycle,while 34.7% of the transgenic soybean could not only germinate and grow up,but also could amplify the right specific segment using CaMV35S and bar primers.We can deduce that the germination method is a rapid and effective method to detect glufosinate-tolerant transgenic soybean.

References:

[1]张文银,安永平,王彩芬,等.主要转基因作物研究现状及其产业化进展[J].生物技术通报,20085:1-4.(Zhang W YAn Y PWang C F,et al.Review of development of genetically modified crops on research and commercial use[J].Biotechnology Bulletin,20085:1-4.)

[2]刘海坤,卫志明.大豆遗传转化研究进展[J].植物生理与分子生物学学报,200531(2):126-134.(Liu H K,Wei Z M.Recent advances in soybean genetic transformation[J].Journal of Plant Physiology and Molecular Biology,200531(2):126-134.)

[3]张宏军,崔海兰,倪汉文,等.抗草胺膦转基因水稻快速方法比较[J].生物技术通报,2003(1):45-48.(Zhang H J,Cui H L,Ni H W,et al.Comparison of the methods of detecting glufosinate-tolerant transgenic rice seeds[J].Biotechnology Bulletin,2003(1):45-48.)

[4]徐传祥,蒯本科,王文东,等.简单实用的转抗除草剂基因后代植株鉴定方法-种子萌发测法[J].复旦学报(自然科学版),2000303):318-321.Xu C X,Kuai B K,Wang W D,et al.Seed germination test a simple but effective way of analyzing transgenetic progenies transformed with the bar gene[J].Journal of Fudan University(Natural Science Edition),2000,30(3):318-321.

[5]王关林,方宏筠.植物基因工程原理与技术[M].北京:科学出版社,1998.Wang G L,Fang H J.Plant genetic engineering[M].Beijing:Science Press,1988.

[6]张宏军,刘学,张佳,等.草铵膦的作用机理及其应用[J].农药科学与管理,2004(4):23-27.(Zhang H JLiu XZhang J,et al.Mechanism and utilization of glufosinate-ammonium[J].Pesticide Science and Administration2004(4):23-27.)

[7]张宏军,倪汉文,周志强,等.抗草铵膦转基因作物及其生物安全性研究进展[J].中国农业大学学报,2002,75):54-56.(Zhang H JNi H WZhou Z Qet al.Major progress on biosafety of glufosinate resistant transgenic crops[J].Journal of China Agricultural University20027(5):54-56.)

[8]Thompson C J,Movva N R,Tizard R,et al.Characterization of the herbicide resistance gene bar?from Streptomyces hygrescopicus[J].EMBO Journal,1987,6:2519-2523.

[9]Zhang Z,Xing A,Staswick P E,et al.The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean[J].Plant Cell Tissue and Organ Culture1999,56:37-46.

[10]Zeng P,Vadnais D A,Zhang Z,et al.Refined glufosinate selection in Agrobacterium-mediated transformation of soybean[Glycine max(L.)Merrill][J].Plant Cell Reports,2004,22:478-482.

[11]王清连,张宝红,郭腾龙,等.转基因棉花外源基因的遗传[J].生命科学研究,2001,54):345-350Wang Q L,Zhang B H,Guo T L,et al.Inheritance of exogenous genes in transgenetic cotton[J].Life Science Research,2001,5(4):345-350.

[12]简玉瑜,陈远玲,李静,等.转基因水稻外源基因的遗传和表达初步研究[J].华南农业大学学报,2001,221):56-59.Jian Y Y,Chan Y L,Li J,et al.Primary studies on inheritance and expression of foreign gene in transgenic rice[J].Journal of South China Agriculture University,2001,22(1):56-59.

[13]孙磊,韩俊友,李宏宇.大豆再生植株抗草铵膦的筛选方法研究[J].大豆科学,2010,296):1019-1023.(Sun L,Han J Y,Li H Y.Comparison on methods screening of transgenetic soybean resistant to glufosinate[J].Soybean Science,2010,29(6):1019-1023.)

Memo

Memo:
-
Last Update: 2014-08-18