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Molecular Deversity and Characterization of Nitrite Reductase Genes from Continuous and Rotational Cropping Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年03期
Page:
425-430
Research Field:
Publishing date:

Info

Title:
Molecular Deversity and Characterization of Nitrite Reductase Genes from Continuous and Rotational Cropping Soybean
Author(s):
XIAO Cui-hong1; SUN Dong-mei1; TANG Hui2; LIU Zhen-hua1
1.College of Life Science,Heilongjiang August First Land Reclamation University,Daqing 163319,Heilongjiang;
2.College of Life Science,Hebei University,Baoding 071002,Hebei,China
Keywords:
Continuous cropping; Soybean; Rotational cropping; Nitrite reductase(nirS)
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.018
Abstract:
Gene sequence analysis of nitrite reductases was performed on continuous cropping and rotational cropping soils of soybean to assess their incidence in different bacterial taxa and their taxonomical value.NirS gene fragments could be amplified from both soils.A total of 268 nirS clones were detected by restriction fragment length polymorphism(RFLP)analysis and obtained 29 and 34 distinct nirS clones in continuous cropping and rotational cropping soils respectively.A dominant nirS pattern,a single dominant nirS restriction pattern of rotational cropping,was observed in both samples.Beside above pattern,another two dominant patterns were also detected in continuous cropping soil.Many of the sequences belonged to dominant pattern were not closely related to previously observed genes and some phylogenetically related sequences were obtained from similar samples.The results indicated that the continuous cropping and rotational cropping soils contained novel nirS sequences,functional diversity of nirS genes changed in the two type soils.

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Last Update: 2014-08-16