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Comparison with Cotyledonary Node and Embryonic Tip Regeneration System in Soybean\[Glycine max(L.)Merrill\] and Genetic Transformation of SR1 (PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年03期
Page:
364-367，373
Research Field:
Publishing date:

Info

Title:
Comparison with Cotyledonary Node and Embryonic Tip Regeneration System in Soybean\[Glycine max(L.)Merrill\] and Genetic Transformation of SR1
Author(s):
YAO Bing-chen1; SHEN Yan-ru1; HAN Xue2; WANG Lin-lin1; LIU Chun-yan2; HU Guo-hua2; 3; CHEN Qing-shan1
1.College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang;
2.Crop Research and Breeding Center of Land-Reclamation,Harbin 150090,Heilongjiang;
3.National Research Center of Soybean Engineering and Technology,Harbin 150050,Heilongjiang,China
Keywords:
Soybean; Cotyledonary node; Embryonic tip; SR1; Genetic transformation
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.006
Abstract:
In this study,the soybean cotyledonary node and embryonic tip were used as explants to transform SR1 gene into Hefeng 25 and Hefeng 35,which are susceptible to soybean?Phytophthora root rot.The results showed that the regeneration of Hefeng 35 reached 96.1% and 79.1%,in embryonic tip and cotyledon node system,respectively,which was significantly higher than that of Hefeng 25(66.45% and 74.4%).The mean regeneration period of Hefeng 35 in embryonic tip and cotyledonary node transformation system was 40 and 68 d,respectively.When the explants of the two systems grew on the SIM or SEM for 20 days,the regeneration frequency of embryonic tip(96.1%)was higher than that in cotyledonary node transformation system(77.8%).The SR1 gene was transferred into Hefeng 35 by embryonic tip explants,and obtained 6 transgenic plants.

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Last Update: 2014-08-16