|Table of Contents|

Optimization of Embryonic Tip Regeneration System and Comparison with Cotyledonary Node Regeneration System in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年03期
Page:
353-357
Research Field:
Publishing date:

Info

Title:
Optimization of Embryonic Tip Regeneration System and Comparison with Cotyledonary Node Regeneration System in Soybean
Author(s):
WANG Wei1; WANG Gang1; 2; JI Jing1; 2; ZHENG Li-hong2; GUAN Chun-feng1; WANG Ping3
1.School of Agriculture and Bioengineering,Tianjin University,Tianjin 300072;
2.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072;
3.School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,Jiangsu,China
Keywords:
Soybean; Embryonic Tip; Cotyledonary node; Plant regeneration
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.004
Abstract:
In order to obtain high efficiency soybean regeneration system,the effect of different factors on the shoots induction of embryonic tips was investigated,including soaking time,induction time and 6-BA concentration and compared the optimized embryonic tip regeneration system with cotyledonary node regeneration system.The results indicated that 24 h seed soaking was suitable for adventitious shoots formation,the adventitious shoots induction rate and mean number of shoots could reach 85.1% and 4.9,respectively.The optimum inducing time was 48 h,and the adventitious shoots induction rate and the mean number of shoots were 84.2% and 5.3,respectively.And the optimal concentration of 6-BA in induction medium was 2.0-3.0 mg·L-1.In comparison with cotyledonary node regeneration system,embryonic tip regeneration system was obviously superior in regeneration capacity and culture period，except for rooting rate which had no significant difference.The embryonic tip regeneration system from Jiyu 91 was more suitable for receptor materials in genetic transformation of soybean.

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Last Update: 2014-08-16