|Table of Contents|

A Simple Soybean in Planta Transformation Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年03期
Page:
347-352,357
Research Field:
Publishing date:

Info

Title:
A Simple Soybean in Planta Transformation Method
Author(s):
GUO Bing-fu1; 2; LIU Jie1; 2; HONG Hui-long2; QIU Li-juan2
1.College of Life Sciences,Nanchang University,Nanchang 330031,Jiangxi;
2.The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/MOA Key Lab of Soybean Biology(Beijing),Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China
Keywords:
Soybean; Agrobacterium; Epicotyl tip; In planta transformation
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.003
Abstract:
In this paper, the terminal bud of germinated soybean seedlings were longitudinal cut and infected by Agrobacterium firstly, and then transplanted the seedlings into field. Eliminated the seedlings without branches on main stem, transformed plants leaves were painted or sprayed with 100 mg·L-1 glufosinate, 3 days later observed leaves reaction and accounted for the resistant plant number. All alive plants were identified by PCR and analyzed the expression of EPSPS gene in T2 generation. There were 75 and 65 plants with resistance to 100 mg·L-1 glufosinate in T0 and T1 generation, respectively; 6 and 52 positive plants in T0 and T1 generation by sequencing the PCR product, respectively; the result showed that EPSPS gene could inherited in offspring. This transgenic method could short transformation cycle, enhance efficacy and provide reference for the optimization and improvement of Agrobacterium transformation.

References:

[1]Kereszt A,Li D G,Arife I,et al.Rhizogenes-mediated transformation of soybean to study root biology[J].Nature Protocols,2007,2:948-952.

[2] 吴颖，王萍，刘海学，等.农杆菌介导的大豆遗传转化[J].内蒙古民族大学学报（自然科学版），2003，18（3）：235-240.(Wu Y,Wang P,Liu H X,et al.Agrobacterium-mediated transformation in soybean[J].Journal of Inner Mongolia University for Nationalities,2003,18(3):235-240.)

[3] 强胜，宋小玲，戴伟明.抗除草剂转基因作物面临的机遇与挑战及其发展策略[J].农业生物技术学报，2010，18（1）：114-125.(Qing S,Song X L,Dai W M.The opportunity and challenge faced by transgenic herbicide-resistant crops and their development strategy[J].Journal of Agricultural Biotechnology,2010,18(1):114-125.)

[4] Hinchee M A W,Connor D D,Newen C A,et al.Production of transgenic soybean plants using Agrobacterium-mediated genetic transfer[J].Biotechnology,1988,6:915-922.

[5] Macabe D E,Swain W F,Martinen B J,et al.Stable transformation of soybean(Glycine max)by particle acceleration[J].Biotechnology,1988,6:923-929.

[6] Townsend J A,Thomas L A.An improved method ofAgrobacterium-mediated transformation of cultured soybean cells:USA,WO94/02620[P].1993.

[7] 徐香玲，高晶，刘伟华，等.Ti质粒介导的B、t、k-δ内毒素蛋白基因转化大豆的初步研究[J].大豆科学，1997，16（1）：6-11.(Xu X L,Gao J,Liu W H,et al.Studies on transferring Bt k-delta endotoxin gene into soybean with Ti-plasmid prmarl[J].Soybean Science,1997,16(1):6-11.)

[8] 党尉，卫志明.根癌农杆菌介导的高效大豆遗传转化体系的建立[J].分子细胞生物学报，2007，40（3）：185-195.(Dang W,Wei Z M.Efficient?Agrobacterium-mediated transformation of soybean[J].Journal of Molecular Cell Biology,2007,40(3):185-195.)

[9] Dang W,Wei Z M.An optimized Agrobacterium-mediated transformation for soybean for expression of binary insect resistant genes[J].Plant Science,2007,173:381-389.

[10]王晓春，王罡，王萍，等.农杆菌介导法转化大豆体细胞胚获得转基因植株[J].中国农学通报，2006，22（4）：40-43.(Wang X C,Wang G,Wang P,et al.Genetic transformation of somatic embryos viaAgrobacterium tumefaciensin soybean[J].Chinese Agricultural Science Bulletin,2006,22(4):40-43.)

[11]王晓春，刘尚前，季静，等.农杆菌介导的大豆体细胞胚遗传转化体系的优化研究[J].大豆科学，2006，25（3）：200-204.(Wang X C,Liu S Q,Ji J,et al.Optimization of genetic transformation system in somatic embryos of soybean mediated byAgrobacterium tumefacien[J].Soybean Science,2006,25(3):200-204.)

[12]王萍，郭永来，高世庆，等.基因枪法将GmDREB基因导入大豆的研究[J].大豆科学，2007，26（3）：315-318.(Wang P,Guo Y L,Gao S Q,et al.Transformation GmDREBgene into soybean via particle bombardment[J].Soybean Science,2007,26(3):315-318.)

[13]Yang S H,Li G L,Li M,et al.Transgenic soybean with low phytate content constructed byAgrobacteriumtransformation and pollen-tube pathway[J].Euphytica,2011,177:375-382.

[14]Li S L,Redei G P.Estimate of mutation rate in autogamous diploids[J].Radiation Botany,1969,9(2):125-131.

[15]Mysore K S,Kumar C T R,Gelvin S B.Arabidopsisecotypes and mutants that are recalcitrant to Agrobacterium?root transformation are susceptible to germ-line transformation[J].The Plant Journal,2000,21(1):9-16.

[16]Feldman K A,Marks M D.Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana:A non-tissue culture approach[J].Molecular and General Genetics,1987,208:1-9.

[17]Chee P P,Fober K A,Slightom J L,et al.Transformation of Soybean(Glycine max)by infecting germinating seeds withAgrobacteriumtumefaciens[J].Plant Physiology,1989,91:1212-1218.

[18]Chowrira G M,Akella V,Fuerst P E,et al.Transgenic grain legumes obtained by in planta electroporation-mediated gene transfer[J].Molecular Biotechnology,1996,5(2):85-96.


Memo

Memo:
-
Last Update: 2014-08-16