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Heat Resistance Evaluation of GmHSFA1 Transgenic Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年03期
Page:
341-346
Research Field:
Publishing date:

Info

Title:
Heat Resistance Evaluation of GmHSFA1 Transgenic Soybean
Author(s):
WU Guang-xi1; 2; LIU Li-jun1; YANG De-guang2; TANG Xiao-fei1; WEI Lai1; WANG Peng-fei2;  WANG Xing-yu2
1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086，Heilongjiang;
2.College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
Keywords:
Soybean; Transgenic; Heat shock transcription factors; Heat shock protein; Heat stress
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.002
Abstract:
In this study,the expression of GmHSFA1，GmHSP70，GmHSP22 and GmHSP17.9 in 11 GmHSFA1 transgenic soybean lines and their receptor were evaluated and researched the change in physiological indices(Pro,MDA,Wss),photosynthetic characteristics(Pn,Cond,Ci,Tr)and yield after high temperature treatment.In most of the transgenic soybean lines,the expression of GmHSFA1、GmHSP70、GmHSP22 and GmHSP17.9 were higher than non-transgenic receptor at 28℃ and 48℃.After heat treatment,the increment of proline content and soluble sugar in most transgenic soybean lines were clearly higher than non-transgenic receptor，increment of MDA content were lower than receptor except HTH-4，the decrement of photosynthetic traits and yield in most of the transgenetic materials were less than non-transgenic receptor.Through gray relation analysis,we concluded that the heat resistance of 9 GmHSFA1 transgenic lines were improved significantly than non-transgenic receptor.

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Last Update: 2014-08-16