|Table of Contents|

Comparisons and Analyses on the Two Methods of Evaluating Resistance to Soybean Cyst Nematode(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年02期
Page:
274-277
Research Field:
Publishing date:

Info

Title:
Comparisons and Analyses on the Two Methods of Evaluating Resistance to Soybean Cyst Nematode
Author(s):
GAO Yuan; CHANG Wei; HAN Ying-peng; LI Wen-bin
Soybean Research Institute,Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry,Harbin 150030,Heilongjjiang,China
Keywords:
Soybean Cyst Nematode; Resistance evaluation; Plastic column method; Acid magenta dyeing method
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.02.023
Abstract:
The difference of two identification method of soybean cyst nematode including plastic column method and acid magenta dyeing method were compared from aspects of cyst counting methods，soybean planting method，and identification accuracy.For plastic column method,the typical symptom of infected plants including yellowed leaves,dwarfed plants and formation of cyst in root could be seen directly.For acid magenta dyeing method,the number of second-stage juveniles (J2) of SCN could be counted under the 20×microscope,while the morphological variation couldn’t be seen.The two methods had the same ability to identify resistant and susceptible materials,while acid magenta dyeing method showed higher efficiency and accuracy.Results suggest when identifying SCN resistance for larger soybean population,acid magenta dyeing method is suitable for its time-saving and high-efficiency characteristics.

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Last Update: 2014-08-15