Effects of Sulfur-rich Amino Acids Transgenic Soybeans on Soil Organic Elements and Microbial Community Diversity(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年02期

Page:

250-265

Research Field:

Publishing date:

Info

Title:

Effects of Sulfur-rich Amino Acids Transgenic Soybeans on Soil Organic Elements and Microbial Community Diversity

Author(s):

CHEN Feng¹; PENG Xin¹; HUA Xiao-mei²; ZHANG Pei-pei¹; GUI Heng¹; QI Jin-liang¹; YU De-yue³; YANG Yong-hua¹

1.State Key Laboratory of Pharmaceutical Biotechnology,School of Life Science,NJU-NJFU Plant Molecular Biology Institute,Nanjing University,Nanjing 210093;

2.Nanjing Institute of Environmental Sciences,SEPA,Nanjing 210042;

3.National Center for Soybean Improvement,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China

Keywords:

Transgenic soybean; Organic element; Biolog; Soil microbes

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.02.020

Abstract:

In this study,the effects of sulfur-rich amino acids transgenic soybeans on soil organic elements and functional diversity of microbial community were investigated to reveal the safety of transgenic soybeans on soil ecology environment.Microorganism community of transgenic soybeans(Group A:three transgenic soybean lines OE-8,OE-7,RNAi-3 and their recipient Nannong 88-1;Group B:three transgenic soybean lines Gagal 17-4,Gagal 21-8,Gagal 57 and their recipient N2899)in mature stage were analyzed by Biolog ECO/GN/GP/FF systems.The results showed that sulfur contents in rhizosphere soil were significantly decreased in the soil of all transgenic soybean lines(P＜0.01).There were also differences in activities,community diversities and richness of soil microbial between transgenic soybeans and non-transgenic soybeans.Two groups of soybeans showed different patterns because of their different recipient genotypes.In Group A,average well color developments(AWCDs)and McIntosh indexes of transgenic soybeans were lower than CK1,especially AWCDs of gram-negative bacteria,and AWCDs,McIntosh indexes of gram-positive bacteria.In rhizosphere soil of three transgenic soybeans,AWCDs of gram-negative bacteria were significantly lower than Nannong88-1(P＜0.01),and McIntosh index of gram-positive bacteria were significantly lower than Nannong88-1(P＜0.05).McIntosh index of fungi in rhizosphere soil of transgenic soybean OE-8 was significantly lower than Nannong88-1(P＜0.01).In Group B,AWCDs of gram-positive bacteria of Gagal 17-4 and Gagal 21-8 were significantly higher than N2899,while AWCD of gram-positive bacteria of Gagal 57 was significantly lower than N2899(P＜0.05).As for both groups,there were no significant differences in whole microorganisms when we compared AWCDs and indexes of community diversity in Biolog ECO plates.These results suggested that transgenic soybeans in our study could change activities of gram-negative and gram-positive bacteria,community diversities of gram-positive bacteria and fungi,and at the same time depended on the genotypes of recipients.

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