Selection of Suitable Soybean Genotype Based on Cotyledon Node and Embryonic Tip Regeneration Systems(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年02期

Page:

212-216

Research Field:

Publishing date:

Info

Title:

Selection of Suitable Soybean Genotype Based on Cotyledon Node and Embryonic Tip Regeneration Systems

Author(s):

ZHENG Li-hong¹; 2; JI Jing²; WANG Gang²; GUAN Chun-feng²; WANG Ping³; WANG Wan-wan¹; 2

1.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072;

2.Tianjin Research Institute of Genetic Engineering,Tianjin 300072;

3.School of Marine Science and Technology,Huaihai Institute of Technology, Lianyungang 222005， Jiangsu,China

Keywords:

Soybean; Genotype; Cotyledon node; Embryonic tip; Regeneration system

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.02.011

Abstract:

Cotyledon nodes and embryonic tips of soybean were used as explants to investigate the induction rate of adventitious shoots,elongation rate of adventitious shoots and the number of adventitious shoots.The results showed that genotype could affect regeneration frequency obviously in the selected regeneration system.For Hefeng35 and Hefeng25,both induction rates and elongation rates in cotyledon node regeneration system were higher than those in embryonic tip regeneration system,while the induction rates and elongation rates of Heinong37 were opposite.Different regeneration system should be applied when different soybean genotypes were used.Hefeng35,Hefeng25 and Suinong14 were suitable for cotyledon node regeneration system,Heinong37 was more suitable for the embryonic tip regeneration system.Furthermore,Jiyu91 and Beijingxiaolidou were suitable for both regeneration systems.

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