|Table of Contents|

Optimization on Genetic Transformation Conditions of Agrobacterium-mediated Cotyledonary-nodes from Different Genotype Soybeans(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年02期
Page:
188-192
Research Field:
Publishing date:

Info

Title:
Optimization on Genetic Transformation Conditions of Agrobacterium-mediated Cotyledonary-nodes from Different Genotype Soybeans
Author(s):
YANG Zhe1; TANG Xiao-fei1; ZHANG Ying1; DONG Xing-yue1; WANG Feng-yi2; LIU Li-jun1
1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086;
2.College of Agriculture，Northeast Agricultural University,Harbin 150030,Heilongjiang,China
Keywords:
Soybean;  Different genotype; Agrobacterium-mediated; Conditions for genetic transformation
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.02.006
Abstract:
Cotyledonary-nodes of Heinong 46,Heinong 53 and Heinong 56 were used as transformation receptors.Culture conditions including 6-BA concentration,glufosinate concentration,infection time,co-culture time and type of elongation medium were screened,in order to find the optimal conditions for Agrobacterium-mediated genetic transformation from different soybean genotypes.Result showed the optimal 6-BA induction concentrations for Heinong 46,Heinong 53 and Heinong 56 were 1.7,1.6 and 1.7 mg·L-1,respectively;the optimal concentrations of glufosinate for Heinong 46,Heinong 53 and Heinong 56 were 2.0,3.0,and 2.0 mg·L-1,respectively;the optimal combination of infection time,co-culture time and type of elongation medium was infection time 25 min,co-culture time 3 d and type Ⅲ of elongation medium.

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Last Update: 2014-08-15