|Table of Contents|

Induction of Multiple Shoots from Soybean Cotyledonary Node(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年02期
Page:
184-187,192
Research Field:
Publishing date:

Info

Title:
Induction of Multiple Shoots from Soybean Cotyledonary Node
Author(s):
SUN Xin; YAN Fan; ZHAO Jian-ru; CHEN Hong-di; ZHAI Ying; WANG Ying; LI Jing-wen; WANG Qing-yu
College of Plant Science,Jilin University,Changchun 130062,Jilin,China
Keywords:
Soybean cotyledonary node; Regeneration; Multiple shoots; Jilin 47
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.02.005
Abstract:
In order to establish a cotyledonary node regeneration system for soybean(Glycine max L.),the cotyledonary node of 4 soybean genotypes were chosen as explants and Jilin 47,which had the maximum induction rate of multiple shoots, was screened out.Then Jilin 47 was used as explants to study the effect of sterilization hours,germination condition,concentration of auxin on induction rate,elongation rate and average shoot.When sterilized for 6-10 h and germinated 5-7 days in 16 h/8 h photoperiod,Jilin 47’s germination,shoot induction and elongation rate were higher than other treatments.The best hormone combination for multiple shoots induction were 2 mg·L-16-BA and 0.1 mg·L-1 IBA,and that for shoots elongation were 0.3 mg·L-1 IAA and 0.5 mg·L-1 GA.

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Last Update: 2014-08-15