Genes on Phenylalanine Acid Metabolic Pathway in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年02期

Page:

178-183

Research Field:

Publishing date:

Info

Title:

Genes on Phenylalanine Acid Metabolic Pathway in Soybean

Author(s):

DU Xiang-yu¹; LIU Chun-yan²; WU Qiong¹; JIANG Hong-wei²; XIN Da-wei¹; CHEN Qing-shan¹; 3; LUAN Huai-hai²; HU Guo-hua²; 3

1.College of Agriculture,Northeast Agricultural University,Harbin 150030;

2.The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090;

3.The National Research Center of Soybean Engineering and Technology,Harbin 150050,Heilongjiang,China

Keywords:

Soybean; Phenylalanine acid; Gene; Mapping; Gene structure

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.02.004

Abstract:

Synthesis of key enzyme genes on protein metabolic in soybean is quantitative traits controlled by multiple genes.Genetic markers and structural information with associated genes of plant protein synthesis can be obtained through mining the information of the key enzyme on protein synthesis in Arabidopsis.In this research,the soybean public genetic maps and physical maps ware integrated,and the key enzyme gene sequences were blasted with soybean genome database by local blast software.Nine key enzyme genes in phenylalanine acid metabolic pathway of soybean were mapped on soybean genetic map.The result indicated that these 9 enzyme genes were mapped on 13 linkage groups,including D1a,B1,N,I,O,C1,C2,F,L,A2,J,D1b,and B2,respectively.And the flanking markers of these genes on the linkage group were obtained.Furthermore,the sequence information between cDNA and gDNA was compared,the number of exon was from 5 to 12,and the number of intron was from 4 to 11.The corresponding markers obtained from the mapping are available for molecular assisted selection,and the structure information can be used in further gene function analysis.

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