|Table of Contents|

A Preliminary Study on Transferring Δ6-Fatty Acid Desaturase Gene from Borago Officinalis into Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年02期
Page:
173-177
Research Field:
Publishing date:

Info

Title:
A Preliminary Study on Transferring Δ6-Fatty Acid Desaturase Gene from Borago Officinalis into Soybean
Author(s):
SONG Li-ya; GE Yan; HE Cong-fen
Beijing Technology and Business University,Beijing Key Laboratory of Plant Resources Research and Development，Beijing 100048,China
Keywords:
γ-linolenic acid; Soybean; Δ6-fatty acid desaturase gene; Agrobacterium mediated
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.02.003
Abstract:
γ-linolenic acid is an essential polyunsaturated fatty acid in human metabolism.Δ6-fatty acid desaturase(D6D)is the key enzyme that can convert linoleic acid into γ-linolenic acid in vivo.In this study,the Δ6-fatty acid desaturase gene from Borago officinalis was amplified by RT-PCR.Sequence analysis showed that the full cDNA contained 1 359 nucleotides.The homology of Borago officinali ?D6D gene in China and that registered in Texas USA was 99%.Recombinant vector pPTN-D6D was constructed that containing the Δ6-fatty acid desaturase gene from Borago officinalis and 35S promoter from plasmid pRTL2.The gene was transferred into two soybean cultivars,Xiaolidou and Kennong18, with Agrobacterium mediated cotylendon node transformation system.The results of PCR indicated that the D6D gene had been introduced and integrated into soybean genome which laid a foundation for soybean quality improvement.

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Last Update: 2014-08-15