Optimization of Agrobacterium-mediated Soybean Transformation using the Cotyledonary Node(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年02期

Page:

167-172

Research Field:

Publishing date:

Info

Title:

Optimization of Agrobacterium-mediated Soybean Transformation using the Cotyledonary Node

Author(s):

HAO Rong-hua¹; SHAO Qun¹; YANG Su-xin¹; LI Ji-cun²; FENG Xian-zhong¹; ZHAO Yan-xiu¹

1.Key Laboratory of Systems Biology in Universities of Shandong,College of Life Science,Shandong Normal University,Jinan 250014;

2.Economic Plants Institute,Jining Academy of Agricultural Sciences,Jining 272031,Shandong,China

Keywords:

Soybean; Agrobacterium tumefaciens; Cotyledonary node; Transformation

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.02.002

Abstract:

To optimize the system of Agrobacterium-mediated soybean\[Glycine max (L.) Merrill\]transformation,effects of 13 cultivars and 2 selection agents were studied during the process of shoot regeneration and transformation.The results showed that the cultivar ‘Shanning 14’ induced the highest number of multiple shoot among tested 13 cultivars,and hygromycin B was better than glufosinate during transformant selection of cultivar ‘Shanning 14’.The optimal transformant selection scheme was a gradient concentration of hygromycin B from 3 to 8 mg·L^-1.The integrations of UidA(GUS)gene into the transformant genome were demonstrated by histological staining,PCR application and sequencing analysis.Transformation efficiency was 3.2% based on the number of transformed events.

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