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A New Soybean Transformation Method Based on Adventitious Shoots Induction(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年02期
Page:
163-166,172
Research Field:
Publishing date:

Info

Title:
A New Soybean Transformation Method Based on Adventitious Shoots Induction
Author(s):
LI Yong-guang; LI Dong-mei; LI Wei-na; GAI Jiang-nan; WANG Tao; GU Han-qing; LI Wen-bin
Soybean Research Institute,Northeast Agricultural University,Chinese Education Ministry’s Key Laboratory of Soybean Biology,Agricultural Ministry’s Northeast Area Key Laboratory of Soybean Biology and Genetic Breeding,Harbin 150030,Heilongjiang,China
Keywords:
Soybean[Glycine max (L.)Merr.]; Transformation method; Agrobacterium tumefaciens; Adventitious shoots induction
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.02.001
Abstract:
In this work,a novel Agrobacterium-mediated transformation methodology,independent of organ culture,was established.The germinated entire seedlings of soybean were used as explants.The transformation system maximum to maintain the integrity of soybean itself,keep off the limitations of traditional tissue culture.The transformation was under non-sterile environment.The transgenic regeneration plants were selected with herbicide glufosinate.The transgenic lines were confirmed by PCR,RT-PCR,qPCR and GUS stainig.Sixteen genotypes of soybean were transformed,and the average transgenic frequency was 23%.Transgenic soybean plants were usually produced in less than 2 month after Agrobacterium inoculation and seeds could be harvested in 3-4 months.The growth characteristics and morphology of transgenic plants were identical to the untransformed wild type plants.This method would be beneficial to facilitate the characteristic of gene functions and boosted the manipulation of soybean transformation for commercial purpose.

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Last Update: 2014-08-15