Induction of Soybean Somatic Embryos and Their Tolerance to Glyphosate(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年01期

Page:

152-154

Research Field:

Publishing date:

Info

Title:

Induction of Soybean Somatic Embryos and Their Tolerance to Glyphosate

Author(s):

WANG Ping¹;  ZHONG Ying¹; 2;  WANG Gang²;  JI Jing²; WANG En-xu³

1.School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005，Jiangsu;

2.School of Agriculture and Biological Engineering,Tianjin University,Tianjin 300072;

3.Agriculture and Parergon Base,Unit of 92323,PLA,Lianyungang 222247,Jiangsu,China

Keywords:

Soybean; Genotype; Somatic embryo; Glyphosate

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.01.035

Abstract:

Immature cotyledons of Hefeng 25,Jilin 35 and Jiyu 91 were used as explants to study the effects of genotypes on the somatic embryogenesis of soybean.Tolerance of soybean immature cotyledons to glyphosate in three genotypes was also investigated.Induction rate of calli in three genotypes raised to 100%.The induction rate of somatic embryogenesis ranged from 53.95% to 72.12% among three genotypes and the average number of somatic embryo in Jiyu 91 was higher than Hefeng 25 and Jilin 35.The induction rate of somatic embryogenesis varied with glyphosate concentrations.The results suggest that somatic embryogenesis should be selected by using 2.5-5.0 mg·L^-1 of glyphosate for transformation with immature cotyledons as explants in soybean.

References:

[1]刘定富.美国主要作物转基因商业化进展及启示[J].中国种业,2010(12):22-25.（Liu D F.Progress and implications for major commercial crops modified genetically in USA[J].China Seed Industry,2010(12):22-25.）

[2]Hinchee M A W,Conner-Ward D V,Newell C A,et al.Production of transgenic soybean plants using Agrobacterium—mediated DNA transfer[J].Nature Biotechnology,1988,6:915-922.

[3]Ranch J P,Oglesby L,Zielinski A C.Plant regeneration from embryo-derived tissue cultures of soybeans[J].In Vitro Cellular & Developmental Biology,1985,21(11):653-658.

[4]Lazzeri P A,Hildebrand D F,Collins G B.Soybean somatic embryogenesis:effect of hormones and culture manipulations[J].Plant Cell Tissue and Organ Culture,1987,10:197-208.

[5]Komatsuda T,Ohyama K.Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max[J].Theoretical and Applied Genetics,1988,75:695-700.

[6]冯新华,蒋兴邨,邵启全.大豆 [Glycine max(L.)Merr.]未成熟子叶组织的体细胞胚胎诱导和植株再生的研究[J].中国科学（B辑）,1988(9):939-943.（Feng X H,Jiang X C,Shao Q Q.Induce of somatic embryo and plant regeneration from immature cotyledons of soybean[J].Science in China(Series B),1988(9):939-943.）

[7]周思君,尹光初,雷勃钧,等.从大豆幼胚诱导胚胎发生再生植株[J].大豆科学,1989,8(1):39-45.（Zhou S J,Yin G C,Lei B J,et al.Plant regeneration from immature embryo culture of soybean via somatic embryogenesis[J].Soybean Science,1989,8(1):39-45.）

[8]隋德志,王连铮,尹光初,等.大豆体细胞组织培养再生植株的研究Ⅰ培养基、基因型、植物激素对诱导大豆再生植株的影响[J].大豆科学,1989,8(2):145-152.(Sui D Z,Wang L Z,Yin G C,et al.A study of soybean plant regeneration via somatic tissue cultures I[J].Soybean Science,1989,8(2):145-152.）

[9]Komatsuda T,Kaneko K,Oka S.Cell biology and molecular genetics genotype×sucrose interactions for somatic embryogenesis in soybean[J].Crop Science,1991,31(2):333-337.

[10]周思君,尹光初,雷勃钧,等.大豆体细胞胚体胎发生影响因素的研究[J].植物学通报,1992,9（2）:38-43.（Zhou S J,Yin G C,Lei B J,et al.Study on the influential factors of the somatic embryogenesis of soybean[J].Chinese Bulletin of Botany,1992,9(2):38-43.）

[11]Jia L,Elizabeth A G.Comparison of somatic embryogenesis and embryo conversion in commercial soybean cultivars [J].Plant Cell Tissue and Organ Culture,1996,44:87-89.

[12]Ranjitha K B D,Settu A,Sujatha G.Somatic embryogenesis and plant regeneration in soybean [Glycine max(L.)Merr.] [J].Indian Journal of Biotechnology,2006，5:243-245.

[13]王萍,王罡,季静,等.大豆体细胞胚胎发生与农杆菌介导的遗传转化[J].遗传,2004,26(5):695-700.（Wang P,Wang G,Ji J,et al.Studies of somatic embryogenesis and genetic transformation by Agrobacterium-mediated in soybean[J].Herditas,2004,26(5):695-700.）

[14]Ko T S,Nelson R L,Korban S S.Screening multiple soybean cultivars(MG 00 to MG VIII)for somatic embryogenesis following Agrobacterium-mediated transformation of immature cotyledons[J].Crop Science,2004,44(5):1825-1831.

[15]Clemente T E,LaVallee B J,Howe A R,et al.Progeny analysis of glyphosate selected transgenic soybeans derived from Agrobacterium-mediated transformation[J].Crop Science,2000,40(3):797-803.

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