Properties of Soybean Proteins Having Affinity Adsorption with Nattokinase(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年01期

Page:

131-134

Research Field:

Publishing date:

Info

Title:

Properties of Soybean Proteins Having Affinity Adsorption with Nattokinase

Author(s):

LI Xi-sheng¹; 2; SHI Yan-mao²; TIAN Zhi-bin¹; 2; DONG Chao²

1.College of Chemistry and Chemical Engineering,Hebei University of Technology,Tianjin 300100;

2.Hebei Institute of Biology,Shijiazhuang 050081,Hebei,China

Keywords:

Nattokinase; Affinity adsorption; Soy proteins; Surface plamon resonance technology

PACS:

TS201.1

DOI:

10.3969/j.issn.1000-9841.2012.01.030

Abstract:

Using Sepharose-4B cross-linked with nattokinase as adsorbing medium,the target proteins were obtained from those of the soybean proteins by affinity chromatography.The molecular weights of target proteins were 30.0 and 26.0 kDa，respectively, measured by degeneration electrophoresis technology.Surface plamon resonance technology was used to determine the affinity adsorption between nattokinase and target soy proteins and the results revealed the protein contains the structure that coupled to nattokinase and the target proteins might be the two subunit pairs of 11S(glycinin).

References:

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