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Technology for Microwave-assistant Extraction of Soyasaponins from Bean Dregs and Its Effects on Tyosinase Activity(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年01期
Page:
112-114,118
Research Field:
Publishing date:

Info

Title:
Technology for Microwave-assistant Extraction of Soyasaponins from Bean Dregs and Its Effects on Tyosinase Activity
Author(s):
LI Ya-jingWU Jian-yangZHENG Ya-ting
Department of Applied Engineering,Zhejiang Economic & Trade Polytechnic,Hangzhou 310018,Zhejiang,China
Keywords:
SoyasaponinMicrowave-assisted extractionTyrosinase activity
PACS:
TS214.2
DOI:
10.3969/j.issn.1000-9841.2012.01.025
Abstract:
Microwave-assistant extraction technology was used to obtain soyasaponins from bean dregs and the optimum technology parameters were determined by orthogonal experiment. Tyrosinase activity was determined by the dopachrome method using L-DOPA as the substrate and the amount of dopachrome in the reaction mixture was measured by spectrophotometer.The optimum technological parameters for soyasaponins extraction were obtained as follow:microwave extraction temperature at 40℃,microwave extraction for 30 min,the solid-liquid ratio 1∶5 and extracted for 3 times.Under above conditions,the soyasaponins content in bean dregs was 8.11%.Soyasaponins had an appreciable antityrosinase activity and the inhibitory effect was enhenced with the concentration increasing,the value of half inhibitory concentration(IC50)was 222.7 μg·mL-1.

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Last Update: 2014-08-15