|Table of Contents|

Influence Factors of Plantlet Regeneration Directly from Cultured Young Embryos in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年01期
Page:
34-37
Research Field:
Publishing date:

Info

Title:
Influence Factors of Plantlet Regeneration Directly from Cultured Young Embryos in Soybean
Author(s):
HE Dao-yi; ZHANG Yan-mei; ZHANG Kun-liang
Anhui Key Laboratory of Resources and Plant Biology，School of Life Sciences，Huaibei Normal University，Huaibei 235000，Anhui，China
Keywords:
Soybean; Young embryo culture; Plantlet
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.01.008
Abstract:
In order to establish the method of plantlet regeneration directly from cultured young embryo of soybean，some influencing factors including embryo age，pre-treatment methods，cultivation medium and soybean genotypes，were investigated respectively.Germination rate of 15 d young embryo was 23%，and that of 20 d or elder young embryo was more than 70%.Although 20 d young embryo could germinate，the development of its bud and root was difficult.Among the 2 days pre-treatment of low temperature（4℃），high temperature（37℃） and GA buffer(100 mmol·L-1)，high-temperature promoted the plantlet rate(75%)of young embryo，while no significant effect were observed for other treatmeats.The tested cultivation medium containing different external hormones had no obvious effect on plant regeneration.The plantlet rate of soybean genotypes varied with maturity groups，and that of extremely early-mature soybean were highest，with Ribenqing and Tainong 292 were 74% and 72%，respectively.

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Last Update: 2014-08-15