|Table of Contents|

Screening of the Optimal Acceptor Genotypes in Introduced Soybean Germplasm(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年01期
Page:
29-33
Research Field:
Publishing date:

Info

Title:
Screening of the Optimal Acceptor Genotypes in Introduced Soybean Germplasm
Author(s):
WU ShuaiWANG Zhi-kunLAN LanZENG Rui SONG BoTUO YunLIU Shan-shan
Soybean Institute, Northeast Agricultural University, Harbin 150030,Heilongjiang,China
Keywords:
SoybeanIntroduced germplasmGenetic transformationGenotype screening
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.01.007
Abstract:
cryI gene was transformed into soybean cotyledon nodes of 6 foreign soybean germplasm by Agrobacterium-midiated method and screened the optimal acceptor genotype by comparing the callus induction,regeneration and etiolate rate.Then the suitable 6-BA concentration for germination and induction mediums of the optimal genotype was studied.The results indicated that the induction,differentiation and etiolation rate were obviously different between soybean genotypes.Among the 6 introduced soybean germplasm,‘Yuehe’ was optimal for tissue culture and genetic transformation.The best hypocotyl-radicel ratio could be obtained when the concentration of 6-BA was 0.1 mg·L-1.The optimal concentration of 6-BA for ‘Yuehe’ transformation was 0.1 and 1.7 mg·L-1in germination medium and bud induction medium,respectively.

References:

[1]李军,李霞,陈杭.大豆茎尖离体培养再生植株[J].植物生理学通讯,2001,37(2):134.(Li J,Li X,Chen H.In vitro culture and plantlet regeneration of shoot tip of soybean[J].Plant Physiology Communications,2001,37(2):134.)

[2]王升吉,吴元华,王洪岩,.大豆不同外植体组织培养及再生研究[J].沈阳农业大学学报,1999,30(3):255-259.(Wang S J,Wu Y H,Wang H Y,et al.Studies on regeneration of culture of different exophytes in soybean[J].Journal of Shenyang Agricultural University,1999,30(3):255-259.)

[3]袁鹰,刘德璞,郑培和,.大豆组织培养再生植株研究[J].大豆科学,2001,20(1):9-13.(Yuan Y,Liu D P,Zheng P H,et al.Regeneration of soybean tissue culture[J].Soybean Science,2001,20(1):9-13.)

[4]王萍,张淑珍,李文滨,.大豆不同基因型胚尖不定芽的诱导及对抗生素的敏感性[J].作物杂志,2010(2):50-53.(Wang P,Zhang S Z,Li W B,et al.Induction of adventitious shoots from embryonic tip of different soybean gennotypes and their sensibility to antibiotics[J].Crops,2010(2):50-53.)

[5]林树柱,曹越平,卫志明.根癌农杆菌介导的大豆遗传转化[J].生物工程学报,2004,20(6):817-820.Lin S Z,Cao Y P,Wei Z M.Agrobacterium tumefaciens-mediated genetic transformation of soybean[J].Chinese Journal of Biotechnology,2004,20(6):817-820.)

[6]武小霞,李静,姜成涛,.大豆子叶节再生中植物生长调节剂浓度及基因型筛选[J].中国油料作物学报,2011,33(2):123-129.(Wu X X,Li J,Jiang C T,et al.Optimization of regeneration system from soybean cotyledonary node[J].Chinese Journal of Oil Crop Sciences,2011,33(2):123-129.)

[7]葛玉君,高丽辉,田福东,.7S球蛋白亚基含量变异大豆种质的性状鉴定[J].中国油料作物学报,2008,30(2):174-178.(Ge Y J,Gao L H,Tan F D,et al.Agronomic and quality characteristics of soybean germplasm identification by various subunit deficiency of 7S globulin[J].Chinese Journal of Oil Crop Sciences,2008,30(2):174-178.)

[8]成丹,孔肖菡,杨梦,.6-BA对种子萌发和幼芽生长的作用研究[J].安徽农业科学,2009,37(33):16725-16726.(Cheng D,Kong X H,Yang M,et al.Study on the effects of 6-BA on the seed germination and sprout growth[J].Journal of Anhui Agricultural Sciences,2009,37(33):16725-16726.

[9]Fischer D C,Kogan M,Paxton J.Effect of glyceollin,a soybean phytoalexin,on feeding by three phytophagous beetles(Coleoptera:Coccinellidae?and Chrysomelidae):dose versus response[J].Environmental Entomology,1990,19(5):1278-1282.

[10]Huang A S,Hsieh O A L,Chang S S.Charaterization of the nonvolatile minor constituents responsible for the objectionable taste of defatted soybean flour[J].Food Science,1982,47(1):19-23.

[11]Rivera-Vargas L I,Schmitthenner A F,Graham T L,et al.Soybean flavonoid effects on and metabolism by Phytophthora sojae[J].Phytochemical Analysis,1993,32(4):851-857.

[12]刘珊珊,刁桂珠,王志坤,.中国和越南大豆种质资源贮藏蛋白亚基组成的鉴定[J].中国油料作物学报,2008,30(4):511-513.(Liu S S,Diao G Z,Wang Z K,et al.Characterization and evaluation for subunit composition of storage protein in soybean germplasm[J].Chinese Journal of Oil Crop Sciences,2008,30(4):511-513.)

[13]林树柱,曹越平,卫志明,.6-BA诱导大豆子叶节和茎尖出芽的研究[J].上海交通大学学报(农业科学版),2005,23(2):138-142.(Lin S Z,Cao Y P,Wei Z M,et al.Studies on shoots induced by 6-BA from cotyledonary nodes and embryonic tips of soybean[J].Journal of Shanghai Jiaotong University(Agricultural Science Edition),2005,23(2):138-142.)

[14]刘莉,赵桂兰.大豆子叶节组织培养再生研究[J].吉林农业科学,1999,24(5):16-19.(Liu L,Zhao G L.Soybean cotyledon node tissue culture regeneration[J].Journal of Jilin Agricultural Sciences,1999,24(5):16-19.)

Memo

Memo:
-
Last Update: 2014-08-15