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Genetic Stability Analysis of the Fifth Generation of Transgenic Soybeans Expressing γ-linolenic Acid(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年01期
Page:
24-28
Research Field:
Publishing date:

Info

Title:
Genetic Stability Analysis of the Fifth Generation of Transgenic Soybeans Expressing γ-linolenic Acid
Author(s):
CHEN Sheng1; GUO Li-qiong1; 2; SONG Jing-shen1; LIN Jun-fang1; 2
1.College of Food Science,South China Agricultural University;
2.Institute of Biomass,South China Agricultural University,Guangzhou 510642,Guangdong,China
Keywords:
Genetic stability; Transgenic soybeans; γ-linolenic acid; △6-fatty acid desaturase
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.01.006
Abstract:
The genetic stability of foreign gene and separation of △6-fad and bar gene were observed in the 5th generation of transgenic soybeans expressing γ-linolenic acid.By PCR amplification and resistance testing of selective marker gene,the genome of transgenic soybeans TS824 and TS825 were integrated △6-fad gene(marker free); TS81, TS83, TS85,TS87,TS814,TS816 and TS818 were integrated bar gene;TS88,TS89,TS810,TS813,TS815,TS816,TS817,TS819 and TS820 were integrated both △6-fad gene and bar gene;neither △6-fad gene nor bar gene was detected in TS82 and TS86.The results showed that △6-fad gene was stably maintained in the genome of part of transgenic soybeans,and △6-fad and bar gene of transgenic soybeans TS824 and TS825 were separated.

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Last Update: 2014-08-15