|Table of Contents|

Optimization of the Factors Affecting Genetic Transformation of Soybean Cotyledonary Node Mediated by Agrobacterium tumefacines(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年01期
Page:
17-23
Research Field:
Publishing date:

Info

Title:
Optimization of the Factors Affecting Genetic Transformation of Soybean Cotyledonary Node Mediated by Agrobacterium tumefacines
Author(s):
CHEN Li-miao1; 2; 3; TIAN Xing-xing1; 2; SHAN Zhi-hui1; 2; SHA Ai-hua1; 2; LI Wen-bin3; ZHOU Xin-an1; 2
1.Oil Crops Research Institute of Chinese Academy of Agricultural Sciences(CAAS),Wuhan 430062,Hubei；
2.Key Laboratory of Oil Crop Biology,Ministry of Agriculture,Wuhan 430062,Hubei；
3.Key Laboratory of Soybean Biology,Ministry of Education,Soybean Research Institute,Northeast Agriculture University,Harbin 150030,Heilongjiang,China
Keywords:
Cotyledonary node; Embryonic tip; Genotype; A.tumefacines concentration; Infection time; Acetosyringone; Sonication
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.01.005
Abstract:
Three soybean genotypes were used for optimal genotype selection in transformation mediated by Agrobacterium tumefacines.The influences of A.tumefacinesconcentration,infection time,acetosyringone(AS) concentration and sonication treatment were studied in this research.As a result,the appropriate genotype for cotyledonary node transformation was Dongnong 50 while Heinong 41 was fit for embryo tip transformation.In the cotyledonary node transform system,the optimal infection combination was A.tumefacines concentration at OD600=0.6 with 30-40 min infection time.The perfect AS concentration was 200 μmol·L-1.The appropriate sonicate treatment was 5 s under 100 W.The study optimized soybean cotyledonary-node Agrobacterium-mediated transformation system and transformation frequency was 2.3%.

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Last Update: 2014-08-14