Rapid Identification of GmCRY1 and GmCRY2 Function by Leaf Injection in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2012年01期

Page:

13-16

Research Field:

Publishing date:

Info

Title:

Rapid Identification of GmCRY1 and GmCRY2 Function by Leaf Injection in Soybean

Author(s):

QIN Zheng-rui¹; 2; SUN Lei¹; 2; LI Hong-yu²

1.College of Plant Science,Jilin University,Changchun 130062,Jilin；

2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 10081,China

Keywords:

Soybean; Cryptochrome genes; Agrobacterium tumefaciens; Flowering time

PACS:

S565.1

DOI:

10.3969/j.issn.1000-9841.2012.01.004

Abstract:

Soybean(Glycine max)Cryptochrome 1(GmCRY1)and Cryptochrome 2(GmCRY2)genes were constructed into expression vector pEGAD,and injected into soybean leaves by Agrobacterium tumefaciens EHA105 mediated transformation.The Agrobacterium tumefaciens EHA105 with vector pEGAD was used as control.The RT-PCR method was carried on to detect if the GmCRY1 and GmCRY2 were transient expressed in the leaves.The transformed plants were grown in different photoperiod light conditions(SD:8 h light/16 h darkness;LD:16 h light/ 8 h darkness)and the flowering data was calculated to conclude the effect of transient expression of GmCRY1 and GmCRY2 on the flowering time in soybean.The results indicated that the transient expressed GmCRY1 in soybean flowered earlier in LD,and the GmCRY1 expression was detected in the injected leaves through RT-PCR analysis.Although the expression of GmCRY2 was detected,it had no effect on flowering time in both LD and SD conditions.This result confirmed that the leaves injection method can quickly identify the function of GmCRY1 and GmCRY2 genes on soybean flowering time.

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