|Table of Contents|

Selection of High-efficient Regeneration Genotype from Embryonic Tips of Vegetable-type Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2012年01期
Page:
9-12
Research Field:
Publishing date:

Info

Title:
Selection of High-efficient Regeneration Genotype from Embryonic Tips of Vegetable-type Soybean
Author(s):
ZHONG Can1; XIAO Shen-gen1; ZHU Bao-ge3; XUE Ren-gao4; WEI Zhi-ming2; ZHU Mu-lan2
1.College of Horticulture and Gardening,Hunan Agricultural University,Changsha 410128,Hunan;
2.Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,National Center for Plant Gene Research (Shanghai)Chinese Academy of Sciences（CAS）, Shanghai 200032;
3.Institute of Genetics and Developmental Biology,CAS,Beijing 100101;
4.College of Life and Science,Qingdao Agricultural University,Qingdao 266109,Shandong,China
Keywords:
Vegetable-type soybean; Embryonic tips; Selection of genotype; Regeneration
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.01.003
Abstract:
To study the effect of sterilization,pre-culture days,different concentrations of 6-BA and medium formulations on induction and elongation of adventitious buds,embryonic tips from four vegetable-type soybean varieties ‘Jiaoda 05-133’,‘Jiaoda 02-89’,‘Huning 96-10’ and ‘Qingsuerhao’ were used as the initial explants.The results showed that the optimal sterilization was using 0.1% HgCl2for 10 min and 5% NaClO for 5 min.The optimal adventitious buds induction formulation was MSB5base medium with 3.0 mg·L-16-BA,and the optimal pre-culture days of embryonic tips was 2 d.The combination of 1.0 mg·L-16-BA and 0.1 mg·L-1NAA benefited to produce many adventitious buds.The combination of 0.05 mg·L-16-BA and 0.1 mg·L-1IBA benefited to the elongation of adventitious buds.Among the four varieties,the embryonic tips from Jiaoda 05-133 is the best one for regeneration,with 90.86% of adventitious buds induction rate,4.65 of mean number of adventitious buds and 1.38 cm of mean length of adventitious buds after culture for 15 d.

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