|Table of Contents|

Analysis of Isoflavone Content in Cultivated Soybeans[Glycine max(L.)Merr.] in Northeast China and Comparison of Different Determination Methods(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年06期
Page:
979-986
Research Field:
Publishing date:

Info

Title:
Analysis of Isoflavone Content in Cultivated Soybeans[Glycine max(L.)Merr.] in Northeast China and Comparison of Different Determination Methods
Author(s):
ZHANG Hai-jun; WANG Ying; SU Lian-tai; LI Lin; ZHAI Ying; LI Jing-wen; ZHANG Xin-sheng; WANG Qing-yu
College of Plant Science，Jilin University，Changchun 130062，Jinlin，China
Keywords:
Soybean isoflavones; HPLC; Three-wave length UV spectrohometry; Ultraviolet spectrophotometry
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.06.0979
Abstract:
Two different soybean isoflavone determination methods including three-wave length UV spectrohometry(TL-UV)and ultraviolet spectrophotometry(UV)were established.The two methods and high-performance liquid chromatographic method(HPLC)established before were optimized and used to measure 128 soybean varieties from northeast China.Single factor experiment and orthogonal experiment were designed to optimize extracted method of soybean isoflavone as determination by TL-UV and UV using ultrasonic wave.The most suitable condition was extracting soybeans with 70% edible ethanol under the 1∶25 ratio of defatted soybean meal to solvent at 50℃ for 5 h，extracting 2 times.Accuracy and precision of the three methods in a descending order was HPLC，TL-UV，UV.HPLC had high analytic sensitivity and accuracy，and was suitable for determination of isoflavone in soybean and other cereal products.The other two methods were suitable for rapid determination and accurate analysis of isoflavones.The isoflavone content of 128 soybean varieties ranged from 1.0404 to 4.4344 μg·mg-1，and the average content was 2.9174 μg·mg-1.The order of soybean isoflavone content among regions was Heilongjiang>Jilin>other region. Twelve varieties with high-soybean isoflavone exceeded 4.0 μg·mg-1 and 3 varieties with low-soybean isoflavone being inferior to 1.0 μg·mg-1were screened.

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Last Update: 2014-08-18