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Construction and Primary Analysis of a SSH Library of Wild Soybean Induced by SMV(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年06期
Page:
968-971
Research Field:
Publishing date:

Info

Title:
Construction and Primary Analysis of a SSH Library of Wild Soybean Induced by SMV
Author(s):
SHI Feng-yuZHU Ying-boLONG RuWU Yun-pengQIAO Ya-ke
College of Life Science,Hebei Normal University of Science and Technology,Changli 066600,Heibei,China
Keywords:
Wild soybean Soybean Mosaic Virus Suppression subtractive hybridization(SSH) cDNA library
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.06.0968
Abstract:
To isolate the genes related to wild soybean resistance in Soybean Mosaic Virus(SMV),Suppression Subtractive Hybridization(SSH)was utilized to construct the different expression cDNA library using wild soybean variety Bian0526 inoculated with race 3 of SMV.From this library,a total of 15 343 positive clones were screened,and the length of most of the insert fragments was focused on 500-800 bp.ESTs similarity analysis of these unigenes was performed with the Blastx and Blastn against the non-redundance database in GenBank.The results showed that 200 functional ESTs had been gotten,and they were related to cell self-protection and system acquired resistance,etc.This indicated that these genes might be involved in resistance to SMV of wild soybean.

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Last Update: 2014-08-18