Acquisition and Identification of Transgenic Soybean using bar as a Selective Agent(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年06期

Page:

895-900

Research Field:

Publishing date:

Info

Title:

Acquisition and Identification of Transgenic Soybean using bar as a Selective Agent

Author(s):

LU Tao¹; LI Hong-yan¹; ZHANG Jie-qiong¹; HU Xiao-nan¹; SHOU Hui-xia²; TANG Gui-xiang¹

1.Institute of Crop Science，College of Agriculture and Biotechnology，Zhejiang University，Hangzhou 310058；

2.Institute of Plant Science，College of Life Science，Zhejiang University，Hangzhou 310058，Zhejiang，China

Keywords:

bar; Transgenic soybean; Progeny detection; Glufosinate selection

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.06.0895

Abstract:

On the basis of optimized soybean Agrobacterium-tumefaciens mediated transformation system，the trypsin inhibitor gene(sporamin)and chitinase gene（chitinase KDEL）were transformed into soybean cultivar YC-2 using germinated 1-2 days mature cotyledon node as explants and bar gene as a selectable agent in this paper.One hundred and fifteen putative transgenic soybean plants had been got and only 45 plants were positive transgenic plants identified by leaf painting 135 mg·L^-1?Basta and polymerase chain reaction(PCR)methods.Six T₀ positive plants were chosen,and four independent transgenic plants could be inherited into T₁ progeny and the segregation rate of two T₁ progenies were 3∶1，the other two T₁ progenies were more than 3∶1.The results suggest that leaf painting 135 mg·L^-1?Basta could be an effective and costless method to detect genetically modified soybeans progeny with bar gene as a selective agent.

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