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Extraction and Thin Layer Chromatography of Saponins from Wild Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年05期
Page:
857-860
Research Field:
Publishing date:

Info

Title:
Extraction and Thin Layer Chromatography of Saponins from Wild Soybean
Author(s):
ZHANG Qian; LIU Dai-cheng
College of Life Science,Shandong Normal University,Jinan 250014,Shandong,China
Keywords:
Wild soybean; Saponins; Thin layer chromatography(TLC)
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.05.0857
Abstract:
Saponins have many pharmacological actions．The determination of wild soybean saponins contents is the basis of the pharmacological experiments． In this experiment,wild soybean saponins were extracted by Soxhlet extractor with hot circulatory methanol,identified by physical-chemical reactions and the thin layer chromatography(TLC)with the oleanic acid taken as reference standard,and then the TLC scanner was used to determine the content of the saponins.The linear range of wild soybean saponins was from 1 to 5 μg;the average recovery was 98.98% with RSD of 0.41%.The TLC identification was distinct and the spots were clear.The content of saponins in defatted wild soybean was 1.91%,which was significantly higher than that in defatted cultivated soybean.The method is simple and accurate with stable outcomes,and suitable for analysis of wild soybean saponins.

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Last Update: 2014-08-16