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Optimization on the Regeneration System of Soybean Embryonic Tips(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年05期
Page:
757-759,763
Research Field:
Publishing date:

Info

Title:
Optimization on the Regeneration System of Soybean Embryonic Tips
Author(s):
YAN Fan; SUN Xin; ZHAI Ying; CHEN Hong-di; ZHAO Jian-ru; LI Jing-wen; WANG Qing-yu
College of Plant Science,Jilin University，Changchun 130062,Jilin,China
Keywords:
Soybean; Embryonic tip; genotype; Browning rate; Rooting
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.05.0757
Abstract:
In order to screen the optimal genotypes for embryonic tip regeneration system in soybean(Glycine max L.Merr.),the embryonic tips of five soybean cultivars including Jidadou 1 and Jidadou 2,were used as explants to study the effects of 6-benzyl aminopurine(6-BA)concentration on induction and regeneration rate of adventitious bud.The screened soybean cultivar Jidadou 2,fitting for soybean embryonic tip regeneration system,were used as explants to study the influence of Indole-3-butytric acid(IBA)on browning rate of embryonic tips when adding proline and silver nitrate.The browning rate was effectivly inhibited when the concentration of 3.0 mg·L-1 proline and 3.0 mg·L-1silver nitrate were as the additives in co-cultivation medium.Sucrose with a high concentration of IBA could increase the rooting ratio.

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Last Update: 2014-08-15