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Cloning and Expression Analysis of Key Enzymes Genes in Biosynthesis of Soybean Isoflavones(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年05期
Page:
743-748
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Key Enzymes Genes in Biosynthesis of Soybean Isoflavones
Author(s):
QIAN Dan-dan1; GONG De-shun2; JIAO Li1; ZHAI Ying1; ZHANG Hai-jun1; LI Jing-wen1; WANG Ying1; WANG Qing-yu1
1.Plant Germplasm and Application Laboratory,Plant Science College,Jilin University,Changchun 130062,Jilin;?
2.Hefei National Laboratory for Physical Sciences at Microscale,School of Life Science,University of Science and Technology of China,Hefei 230026,Anhui,China
Keywords:
Soybean isoflavones; Chalcone synthase; Chalcone Isomerase; Expression Analysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.05.0743
Abstract:
Chalcone Synthase(CHS)and Chalcone Isomerase(CHI)are two key enzymes in soybean isoflavones biosynthesis pathway,the cDNAs of CHS and CHI were cloned using RT-PCR method and these two genes were fused using SOE-PCR，then constructed E. coli expression vectors to study the expression.The eukaryocyte expression vectors were also constructed and transferred into soybean cotyledonary nodes using Agrobacterium-mediated transformation,and then obtained the resistant transgenic plants.

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Last Update: 2014-08-15