|Table of Contents|

Cloning and Phylogenetic Analysis of Mitochondrial Cytochrome b Gene from Soybean(Glycine max)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年05期
Page:
738-742
Research Field:
Publishing date:

Info

Title:
Cloning and Phylogenetic Analysis of Mitochondrial Cytochrome b Gene from Soybean(Glycine max)
Author(s):
LI Yu-qiu; ZHAO Hong-kun; LIU Xiao-dong; ZHANG Chun-bao; DONG Ying-shan
Biotechnology Research Center，Jilin Academy of Agricultural Sciences，Changchun 130033，Jilin，China
Keywords:
Soybean; Mitochondrial; Cytochromeb
PACS:
Q941
DOI:
10.11861/j.issn.1000-9841.2011.05.0738
Abstract:
In this study，mitochondrial DNA uncompleted sequence was used to design special prime of mitochondrial cytochrome b gene(cob)and obtained conservation domain of soybean cob gene by PCR amplification.The results showed that cob gene was consisted by 1 173 bp which encoded 391 amino acids，with 58.1% A+T and 41.9% G+C content.Southern blot analysis revealed that more than 2 cob gene copies were found in G.max mitochondrial genome.Amino acid sequence and phylogenetic analysis showed that cob was most similar to the dicotyledon，with 98% identity at the nucleotide level.In conclusion，cob gene is a potential hereditary marker for phyletic classification basing on its highly conservative property.

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Last Update: 2014-08-15