Molecular Cloning and Analysis of GmAHRI Gene in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年05期

Page:

727-730,737

Research Field:

Publishing date:

Info

Title:

Molecular Cloning and Analysis of GmAHRI Gene in Soybean

Author(s):

GAO Jing-yao¹;  JIANG Wei¹; LIU Chun-yan²;  JIANG Hong-wei²;  HU Guo-hua²; 3;  CHEN Qing-shan¹; 3

1.College of Agriculture，Northeast Agricultural University，Harbin 150030;?

2.Crop Research and Breeding Center of Land-Reclamation，Harbin 150090;?

3.National Research Center of Soybean Engineering and Technology，Harbin 150030，Heilongjiang，China

Keywords:

Soybean; GmAHRI gene; Sequence analysis

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.05.0727

Abstract:

In this research，the GmAHRI gene was cloned from Glycine max and analyzed its sequences.A cDNA product about 1764 bp was amplified from the total RNA of soybean leaves by reverse transcription PCR(RT-PCR)with specific primers designed with the full-length sequence of GmAHRI gene.Sequence analysis showed that the GmAHRI gene family had two members，named GmAHRI1(FJ594399.1)and GmAHRI2(JN034043)，respectively.The full length of GmAHRI1 and GmAHRI2 were 1764 bp，encoding 587 amino acids，constructed by 10 exons and 9 introns，and located on chromosome Gm12 and Gm13，respectively.The similarity of two genes reached to 96.71% in amino acid level，there were obvious difference in secondary structure，but they had same tertiary structure.

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