|Table of Contents|

Enzyme Activity Analysis of GmLACS in Saccharomyces Cerevisiae(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年05期
Page:
719-722
Research Field:
Publishing date:

Info

Title:
Enzyme Activity Analysis of GmLACS in Saccharomyces Cerevisiae
Author(s):
YU Li-li12TAN Xiao-li2HOU Wen-sheng1
1.Institute of Crop Science,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Beijing 100081;?
2.Institute of Life Sciences,Jiangsu University,Zhenjiang 212013,Jiangsu,China
Keywords:
SoybeanLACSPhylogenic analysisYeast complementation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.05.0719
Abstract:
In this paper,GmLACS was transformed into LACS-deficient Saccharomyces cerevisiae YB525 to analyze its enzymatic activity.Phylogenic analysis indicated GmLACS showed a high degree of homology with other LACSs of plants.GmLACS could complement the YB525 mutant which was deficient in LACS showing that GmLACS belonged to the LACS family.The transformants preferred to use long chain fatty acids indicated GmLACS could activate the free fatty acids and involve the metabolism of fatty acids and lipids.

References:

[1]石彦国.调整产业结构确保大豆产业健康持续发展[J].中国食品学报,2010,10(4):1-6.(Shi Y G.Adjusting the industrial structure to ensure sustained and healthy development of soybean industry[J].Journal of Chinese Institute of Food Science and Technology,2010,10(4):1-6.)

[2]Shockey J M,Fulda M S,Browse J A.Arabidopsis?contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism[J].Plant Physiology,2002,129(4):1710-1722.

[3]Kornberg A,Pricer W E.Enzymatic synthesis of the coenzyme A derivatives of long chain fatty acids[J].Journal of Biological Chemistry,1953,204(1):329-343.

[4]Stuible H P,Kombrink E.Identification of the substrate specificity-conferring amino acid residues of 4-coumarate:coenzyme A ligase allows the rational design of mutant enzymes with new catalytic properties[J].Journal of Biological Chemistry,2001,276(29):26893-26897.

[5]Andrews J,Keegstra K.Acyl-CoA synthetase is located in the outer membrane and Acyl-CoA thioesterase in the inner membrane of pea chloroplaste envelopes[J].Plant Physiology,1983,72(3):735-740.

[6]Fulda M,Shockey J,Werber M,et al.Two long-chain acyl-CoA synthetases from Arabidopsis thaliana?involved in peroxisomal fatty acid beta-oxidation[J].Plant Journal,2002,32(1):93-103.

[7]Fulda M,Shockey J,Werber M,et al.Fatty acid export from the chloroplast.Molecular characterization of a major plastidial acyl-coenzyme A synthetase from Arabidopsis[J].Plant Physiology,2002,129(4):1700-1709.

[8]Shockey J M,Fulda M S,Browse J A.Arabidopsis?contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism[J].Plant Physiology,2002,129(4):1710-1722.

[9]Fulda M,Shockey J,Werber M,et al.Two long-chain acyl-CoA synthetases from Arabidopsis thaliana?involved in peroxisomal fatty acid beta-oxidation[J].Plant Journal,2002,32(1):93-103.

[10]Schnurr J,hockey J,Browse J.The acyl-CoA synthetase encoded by LACS2 is essential for normal cuticle development in Arabidopsis[J].Plant Cell,2004,16(3):629-642.

[11]Lu S,Song T,Kosma D K,et al.Arabidopsis?CER8 encodes long-chain acyl-coA synthetase1(LACS1)that has overlapping functions with LACS2 in plant wax and cutin synthesis[J].Plant Journal,2009,59(4):553-564.

[12]Fulda M,Heinz E,Wolter F P.Brassica napus?cDNAs encoding fatty acyl-CoA synthetase[J].Plant Molecular Biology,1997,33(5):911-922.

[13]Pongdontri P,Hills M.Characterization of a novel plant acyl-coA synthetase that is expressed in lipogenic tissues of Brassica napus?L.[J].Plant Molecular Biology,2001,47(6):717-726.

[14]朱福各,谭小力,崇保强,.油菜脂酰CoA合成酶基因pXT166的鉴定和功能分析[J].中国油料作物学报,200931(3):274-278.(Zhu F G,Tan X L,Chong B Q,et al.Charaterization and functional analysis of pXT166 gene in Brassica napus[J].Chinese Journal of Oil Crop Sciences,2009,31(3):274-278.)

[15]Wang X L,Li X B.The GhACS1 gene encodes an acyl-CoA synthetase which is essential for normal microsporogenesis in early anther development of cotton[J].Plant Journal,2009,57(3):473-486.

[16]He X,Chen G Q,Kang S T,et al.Ricinus communis contains an acyl-CoA synthetase that preferentially activates ricinoleate to its CoA thioester[J].Lipids,2007,42(10):931-938.

[17]Yu L L,Tan X L,Yuan W W,et al.In silicon cloning and analysis of a LACS?gene from Glycine max(L.)[J].International Journal of Biology,2010,2(1):111-116.

[18]Groot P H,Scholte H R,Hülsmann W C.Fatty acid activation:specificity,localization,and function[J].Advances in Lipid Research,1976,14:75-126.

[19]Ohlrogge J B,Kuhn D N,Stumpf P K.Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea[J].Proceedings of the National Academy of Sciences of the United States of America,1979,76(3):1194-1198.


Memo

Memo:
-
Last Update: 2014-08-15