Response of Phenylalanine Ammonia-lyase （PAL）to Different Inducing Conditions in Wild Soybean（Glycine.soja）(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年04期

Page:

703-705,709

Research Field:

Publishing date:

Info

Title:

Response of Phenylalanine Ammonia-lyase （PAL）to Different Inducing Conditions in Wild Soybean（Glycine.soja）

Author(s):

ZHANG Bi-xian¹; 2; HU Xiao-mei¹; ZHU Yan-ming¹; LAI Yong-cai²; 3; LI Wei³; XIAO Jia-lei³; LI Wan³; BI Ying-dong³

1.Life Sciences of Institute of Northeast Agricultural University，Harbin 150030;

2.Postdoctoral Station Heilongjiang Acamedy of Agricultural Sciences， Harbin 150086;

3.Crop Tillage and Cultivation Institute of Heilongjiang Acamedy of Agricultural Sciences，Harbin 150086，Heilongjiang，China

Keywords:

Wild soybean（Glycine soja）; Phenylalanine ammonia-lyase(PAL); Inducing conditions

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.04.0703

Abstract:

Wild Soybean（Glycine soja） with more desired traits is the ancestor of the cultivated soybean（Glycine max）,and phenylalanine ammonia-lyase(PAL)is the key enzyme in the synthesis of isoflavones.With wild soybean(longye 01-177)as material,according to the influencing factors of PAL activities,selecting each 48 h for a cycle and sampling for each 3 h,we studied the enzyme activity,protein content and the variation of enzyme activity under the conditions of low temperature(-4℃)(LT),mechanical damage(MD),ultraviolet ray(UV),hormone(BAP)and normal controls conditions.The results indicated that there was the same change trend of the enzyme activity and protein content of wild soybean PAL under different induction conditions and low temperature had the best induced effect.

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