|Table of Contents|

Determination of Soybean Isoflavones Content by High-Performance Liquid Chromatographic Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年04期
Page:
672-675,679
Research Field:
Publishing date:

Info

Title:
Determination of Soybean Isoflavones Content by High-Performance Liquid Chromatographic Method
Author(s):
ZHANG Hai-jun; SU Lian-tai; LI Lin; LIU Ya-jing; LI Xiao-wei; ZHANG Yan; WANG Ying; LI Jing-wen; WANG Qing-yu
College of Plant Science，Jilin University，Changchun 130062，Jinlin，China
Keywords:
Soybean(Glycine max); High-performance liquid chromatographic method(HPLC); Isoflavone content
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0672
Abstract:
The five soybean isoflavones components including Genistein,Genistin,Daidein,Daidzin and Glycitin were determined and separated by the established HPLC method,and isoflavones in different tissues and different grow stages of embryo were measured.The HPLC condition we have established：an phenomenex C18column(150 mm×4.6 mm,5.0 μm)was applied;the mobile phase was MeOH ∶H2O=30 ∶70;the temperature of column was 40℃;the flow rate was 10 mL?min-1;detective wavelength was set at 254 nm.The peak area of five isoflavones had a good linearity with a correlation coefficient of 0.9994-0.9998,and the recoveries of standard spiling of isoflavones were in the range of 99.29%-100.76% with RSD less than 3%.This method was suitable for determination of isoflavones in soybean and other cereal products.The isoflavone content among soybean varieties was Jilin 32> GR8836>Jidadou 2> Pingan 8> Xiaoheidou>Jilin 47>Jilin 35>Jidadou 2,and soybean isoflavone content of Jilin 32 was that in seeds>60 d embryos>50 d embryos>40 d embryos>flowers>pods>20 d embryos>30 d embryos>leaves>roots>stems.

References:

[1]Wang H J,Murphy P A.Isoflavone content incommercial soybean foods[J].Agricultural and Food Chemistry,1994,42(8):1666-1673.

[2]孙君明,丁安林,张艳,等.大豆异黄酮的研究概况[J].大豆科学,1995,14(2):160-166.(Sun J M,Ding A L,Zhang Y,et al.General review in the study of the soybean isoflavone[J].Soybean Science,1995,14(2):160-166.)

[3]Lo F H, Mak N K,Leung K N.Studies on the anti-tumor activities of the soyisoflavone daidzein on murine neuroblastoma cells[J].Biomedicine & Pharmaeotherapy,2007,61(9):591-595.

[4]Watanabe S,Uesugi S,Kikuchi Y.Isoflavones for prevention of cancer,cardiovascular diseases,gynecological problems and possible immune potentiation[J].Biomedecine﹠Pharmacotherapy,2002,56(6):302-312.

[5]Aedin C,Bryn H,Rosa M.Isoflavones,lignans and stilbenes-origins,metabolism and potential importance to human health[J].Journal of the Science of Food and Agriculture,2000,80:1044-1047.

[6]Gardner C D,Chatterjee L M,Franke A A.Effects of isoflavone supplements vs soy foods on blood concentrations of genistein and daidzein in adults[J].The Journal of Nutritional Biochemistry,2009，20(3):227-234.

[7]王春娥,赵团结,盖钧镒.大豆异黄酮组分HPLC快速分析技术及其在豆腐加工中的应用[J].作物学报,2010,36(12):2062-2072.(Wang C E,Zhao T J,Gai J Y.Establishment of a rapid HPLC method for quantifying isoflavone components and its application in tofu processing[J].Acta Agronomica Sinica,2010,36(12):2062-2072.)

[8]宋志峰,王丽,孟凡钢,等.吉林省普通大豆品种（系）异黄酮含量分析[J].大豆科学,2009,28(6):1076-1080.(Song Z F,Wang L,Meng F G，et al.Analysis of isoflavones content in normal soybean varieties（lines）in Jilin province[J].Soybean Science,2009,28(6):1076-1080.)

[9]孙君明,丁安林,东惠茹.高效液相色谱（HPLC）技术检测大豆异黄酮含量[J].大豆科学,2000,19(1):15-20.(Sun J M,Ding A L,Dong H R.High performance liquid chromatographic dermination of isoflavone content in soybean test sample[J].Soybean Science,2000,19(1):15-20.)

[10]Kudou S,Fleury Y,Welt D,et al.Malony isoflavone glucosides in soybean seeds(Glycine max Merri.)[J].Agricultural and Biological Chemistry.1991,55(9):2227-2233.

[11]孙君明,丁安林,常汝镇,等.中国大豆异黄酮含量的初步分析[J].中国粮油学报,1995,10(4):51-54(Sun J M,Ding A L,Chang R Z,et al.The survey of content of soybean isoflavone in china[J].Journal of the Chinese Cereals and Oils Association,1995,10(4):51-54.)

[12]董李平,吴珍龄,易泽林.大豆结实前异黄酮积累规律的研究[J].西南农业大学学报（自然科学版）,2004,26(6):756-758.(Dong L P,Wu Z L,Yi Z L.Studies on isoflavone distribution and variation before seed-setting in soybean(Glycine Max?L.Merri)[J].Journal of Southwest Agricultural University(Natural Science),2004,26(6):756-758.)

[13]张晓波,吴岩,林红.高效液相色谱法测定水解大豆中异黄酮方法研究[J].粮食与油脂,2006(4):19-21.(Zhang X B,Wu Y,Lin H.Study on method of hydrolyze isoflavone in soybean by HPLC[J].Cereals & Oils,2006(4):19-21.)


Memo

Memo:
-
Last Update: 2014-08-15