|Table of Contents|

Improvement of Two-dimension Electrophoresis for Soybean Leaves Total Protein(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年04期
Page:
663-667
Research Field:
Publishing date:

Info

Title:
Improvement of Two-dimension Electrophoresis for Soybean Leaves Total Protein
Author(s):
GU Xian-peng; LUO Qiu-lan; GAO Ji-guo; LI Wen-bin
Key Laboratory of Soybean Biology of Chinese Education Ministry, Soybean Research Institute, Northeast Agricultural University, Harbin 150030,Heilongjiang,China
Keywords:
Protein extraction; SDS-PAGE; Two-dimension electrophoresis; proteomics
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0663
Abstract:
To improve the two-dimensional polyacrylamide gel electrophoresis(2D-PAGE)technology for separating leaf proteins in soybean,we compared three soybean leaf protein isolation methods,discussed the influence of isoelectric focusing time and staining,chose the different IPG strips to set the electrophoresis program and parameters of the first isoelectric focusing and the second sodium dodecyl sulfate polyacrylamide gel electropheresis(SDS-PAGE).When chose the TCA-acetone precipitation method to extract protein,selected pH 4-7（17 cm）IPG strips,prolonged the isoelectric focusing time of the second and the third step,modified the isoelectric focusing parameters,and combined the coomassie brilliant blue staining and silver staining for 12% gelatin,we obtained clear soybean protein 2D-PAGE map.Results suggest the optimized method is suitable for separating total protein in soybean leaf.

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Last Update: 2014-08-15