A Fast Detection Method for Screening of Gly m Bd 30 K Allergen Protein Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean Seeds(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年04期

Page:

638-642

Research Field:

Publishing date:

Info

Title:

A Fast Detection Method for Screening of Gly m Bd 30 K Allergen Protein Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean Seeds

Author(s):

WANG Shao-dong¹; 3; LI Yuan-ming¹; LIU Wei¹; JIANG Yan¹; WANG Hao¹; YU Jia¹; LI Wen-bin²; TAKAHASHI Koji³

1.National Research Center of Soybean Engineering and Technology(NRCSET),Northeast Agricultural University,Harbin 150030,Heilongjiang,China;?

2.Key Laboratory of Soybean Biology of Chinese Education Ministry,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;

3.National Agriculture and Food Research Organization,National Institute of Crop Science,Tsukuba 305-8518,Ibaraki,Japan

Keywords:

Allergen proteins; Sodium dodecyl sulfate polyacrylamide gel electrophoresis; Western blotting; Soybean seeds

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.04.0638

Abstract:

In this research,through the improvement of the sample pretreatment and confection of the gel,a fast detection method was developed for simultaneous determination of α’,α,β-Subunit and Gly m Bd 30 K lacking individual using sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)in soybean seeds.We have screened F₂(PI603570A×0329F₆)population(N=116)in 7S globuline lacking by this method.Moreover,we have introduced the lacking character into the major soybean varieties in Heilongjiang province through the backcross breeding.The results indicated that the method was better in velocity and less costs of the screening.It is significant for the development of soybean germplasm in the allergen proteins lacking.

References:

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