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Comparison of Three Rapid Gas Chromatograph(GC)Methods for Determination of Fatty Acid Components in Soybean Seeds(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年04期
Page:
626-631
Research Field:
Publishing date:

Info

Title:
Comparison of Three Rapid Gas Chromatograph(GC)Methods for Determination of Fatty Acid Components in Soybean Seeds
Author(s):
YU Fu-kuan; SUN Jun-ming; HAN Fen-xia; GE Yi-nan; ZHANG Jing-ying; MA Lei; ZHANG Jin-wei; YAN Shu-rong; YANG Hua
Institute of Crop Science,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Beijing 100081,China
Keywords:
Soybean[Glycine max(L.）Merr.]; Fatty acids; Gas chromatography(GC)
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0626
Abstract:
Five kinds of fatty acid components including palmitic acid,stearic acid,oleic acid,linoleic acid,linolenic acid,were analyzed by the gas chromatograph(GC)method,and three sample handling methods,including Soxhlet extraction,improved seed-methyl esterification and powder-methyl esterification methods,were compared in the five soybean varieties in order to reveal the differentiation among them.The results showed that the seed-methyl esterification method is simple and fast,which is suitable for the determination of single or half of seed in the low generation.The powder-methyl esterification method is relative accurate,which is good to the quick measurement of a great amount of soybean sample and can avoid decomposing the fatty acid components by the heating for long time.The Soxhlet extraction method has an advantage on the precision of analysis,but needs a great quantity of samples,the long time-consuming and lots of work.The test of precision showed that there was no significant different among the three kinds of methods,which demonstrated that both of the improved seed-methyl esterification and the powder-methyl esterification methods can be useful for determining the fatty acid components in soybean seeds.

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Last Update: 2014-08-15