|Table of Contents|

Transformation of BrCS Gene into Soybean by Agrobacterium-mediated Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年04期
Page:
569-573
Research Field:
Publishing date:

Info

Title:
Transformation of BrCS Gene into Soybean by Agrobacterium-mediated Method
Author(s):
ZHANG Shu-li1; 2; LIU Li-jun1; TANG Xiao-fei1; WEI Lai2
1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086;
?2.College of Agriculture，Northeast Agricultural University,Harbin 150030 Heilongjiang, China
Keywords:
Soybean; Genetic transformation; BrCS gene; Cold-stress tolerance; Salt-stress tolerance
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0569
Abstract:
In this study,plant expression vector pCAMBIA3300-BrCS containing-BrCS-gene was constructed,and transgenic plants were obtained by Agrobacterium-mediated transformation of soybean(Heinong 59 and Heinong53)cotyledonary nodes.We regenerated plants and optimized the conditions of transformation to increase the efficiency of transformation.PCR and PCR-Southern detection was used to identify the integrated level of BrCS gene in transgenic plants;it confirmed the integration of BrCS genes into the genome of soybean.

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Last Update: 2014-08-15