Sensitivity of Different Genotype Soybean Callus to Agrobacterium EHA105(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年04期

Page:

566-568

Research Field:

Publishing date:

Info

Title:

Sensitivity of Different Genotype Soybean Callus to Agrobacterium EHA105

Author(s):

ZHANG Qing-lin¹; ZHAO Yan²; ZHANG Yan¹; ZHAI Ying¹; SU Lian-tai¹; WANG Ying¹; LI Jing-wen¹; WANG Qing-yu¹

1.College of Plant Science,Jilin University,Changchun 130062,Jilin;?

2.College of Life Science and Agro-forestry,Qiqihar University,Qiqihar 161006,Heilongjiang,China

Keywords:

Soybean; Callus; Genotype; GUS staining; Agrobacteriem tumefaciens

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.04.0566

Abstract:

Soybean genotype has great impact on efficiency of genetic transformation.It is easy and convenient to evaluate the transgenic soybean by transient expression of GUS gene.This paper studied the sensitivity of eight genotypes to ?Agrobacterium EHA105 by GUS gene transient expression and staining times of GUS gene in soybean callus.The results showed that the sensitivity to Agrobacterium EHA105 varied with genotypes,the staining of Jilin47 and Dongnong42 were the best among the 8 soybean types indicated that soybean callus of Jilin47 and Dongnong42 were more sensitive to EHA105;the staining time was also varied with genotypes,appropriate GUS staining time was from 9 to 10 h.

References:

[1]王萍,吴颖,季静,等.抗生素对大豆愈伤组织的诱导和生长的影响[J].遗传,2001,23(4):321-324.（Wang P,Wu Y,Ji J,et al.Effect of antibiotics on induction of callus and callus growth in soybean[J].Hereditas,2001,23(4):321-324.）

[2]Hinchee M A W,Connor-Ward D V,Newell C A,et al.Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer[J].Biotechnology,1988,6:915-922.

[3]Hu X W,Liu S X,Guo J C,et al.Embryo and another regulation of the mabinlin Ⅱ sweet protein gene in Capparis masaikai Lévl[J].Functional & Integrative Genomics,2009,9:351-361.

[4]赵艳,史岩玲,钱丹丹,等.大豆脂肪氧化酶-3基因(Lox3)启动子的克隆及其瞬时表达分析[J].生物技术通报,2010,9:65-69.（ZhaoY,Shi Y L,Qian D D,et al.Cloning and transient expression of soybean Lox3?promoter[J].Biotechnology Bulletin,2010,9:65-69.）

[5]汲逢源,王戈亮,许亦农.抗氧化剂对农杆菌介导的大豆下胚轴GUS基因瞬时表达的影响[J].植物生态学报,2006,30(2):330-334.(Ji F Y,Wang G L,Xu Y N.The effects of antioxidants on the transient expression of GUS?gene in soybean hypocotyls mediated by Agrobacterium tumefaciens[J].Journal of Plant Ecology,2006,30(2):330-334.)

[6]Jefferson R A,TKavanagh T A,Bevan W A.β-glucuroonidase as a sensitive and versatile gene fusion marker in higher plants[J].The EMBO Journal,1987,6:3901-3907.

[7]Hinchee M A,Connor W D V,Newell C A,et al.Production of transgenic soybean plants using Agrobacterum-mediated DNA transfer[J].Biotechnology,1988,6:915-922.

[8]Jefferson R A.Assaying chimeric genes in plants:the GUS?gene fusion system[J].Plant Molecular Biology Reporter,1987,5(4):387-405.

Memo

Memo:

-

Common functions