|Table of Contents|

Assessment of Factors Affecting Soybean Cotyledonary-node Agrobacterium-mediated Genetic Transformation(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年04期
Page:
557-562
Research Field:
Publishing date:

Info

Title:
Assessment of Factors Affecting Soybean Cotyledonary-node Agrobacterium-mediated Genetic Transformation
Author(s):
WANG Feng-min; LI Tao; WANG Yun-jie; JING Hui-xian; SHI Xiao-lei; ZHANG Meng-chen; DI Rui; JIANG Chun-zhi
Lab of Crops Genetic Breeding of Hebei,National Soybean Improvement Center Shijiazhuang Sub-Center,Institute of Food and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,Hebei,China
Keywords:
Soybean; GUS;  Agrobacterium tumefaciens strain; Genetypes; Genetic transformation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0557
Abstract:
Based on stability,specificity and transient expression of GUS gene in plant,we assessed factors affecting soybean Agrobacterium-mediated cotyledonary-node genetic transformation including hormone concentrations,selective agent concentration,genotypes,Agrobacterium tumefaciens strain.For shoot induction,a higher regeneration rate was obtained with 1.6 mg?L-16-BA;the suitable selective agent concentration of glufosinate was 3 mg?L-1.Based on their regeneration ability and sensitivity to Agrobacterium EHA101,EHA105 and LBA4404,‘Jilin xiaodou’ and ‘Maverick’ had higher regeneration capacity.The infection rate of ?Agrobacterium strain was EHA101(0-41.03%)better than EHA105(0-35%), LBA4404(0-18.27%)was the lowest.Based on the analysis of receptor genotypes,‘Jilinxiaodou’ and ‘Maverick’ was selected as the best receptor genotypes for the ?Agrobacterium-mediated cotyledonary-node transformation system.When infected by strain EHA101,GUS positive rate of ‘Jilinxiaodou’ and ‘Maverick’ reached 40.25% and 29.89%,respectively;while EHA105 infected soybean cotyledonary-node,percentage of GUS positive of ‘Jilinxiaodou’,‘Maverick’,‘Jidou15’,‘Jidou17’ and ‘nf58’ were 33.36%,27.52%,12.47%,10.67% and 9.11%,respectively.

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