Effect of α-Lipoic Acid on the Agrobacterium-tumefaciens Mediated GUS Transient Expression Rate and Shoot Induction Percentage in Soybean(Glycine max L.)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年04期

Page:

552-556

Research Field:

Publishing date:

Info

Title:

Effect of α-Lipoic Acid on the Agrobacterium-tumefaciens Mediated GUS Transient Expression Rate and Shoot Induction Percentage in Soybean(Glycine max L.)

Author(s):

YANG Xiao-feng¹; LU Tao¹; ZHOU Zheng-jian²; SHOU Hui-xia²; TANG Gui-xiang¹

1.Institute of Crop and Science,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058;

?2.Institute of Plant Science,College of Life Science,Zhejiang University,Hangzhou 310058,Zhejiang,China

Keywords:

Soybean(Glycine max L.); α-Lipoic acid; GUS transient expression; Shoot induction percentage

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.04.0552

Abstract:

Antioxidant-α-lipoic acid(LA)influencing on the Agrobacterium-tumefaciens mediated GUS transient expression rate and shoot induction percentage in soybean cultivar‘YC-2’was studied in this paper.The results showed the explant browning percentage was markedly decreased after added different concentrations of α-lipoic acid in the co-cultivation medium.In the meantime,the explant whitening percentage was increased with the increased concentration of α-lipoic acid in co-cultivation medium.Meanwhile,the whitening explant couldn’t induce shoot regeneration after culured on the shoot induction medium for a month.It also showed the transient GUS expression rate and shoot induction frequency were also increased after added different LA concentrations in the co-cultivation medium.The optimized LA concentration was 25 mg?L^-1in the co-cultivation medium.

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