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High Frequency Induction of Callus from Soybean Cotyledonary Node Explants and Its Plant Regeneration(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年04期
Page:
546-551
Research Field:
Publishing date:

Info

Title:
High Frequency Induction of Callus from Soybean Cotyledonary Node Explants and Its Plant Regeneration
Author(s):
SUN Ming-jie; WEI Xuan; ZOU Dan-dan; ZHAO Chun-mei; CAI Chun-mei; XUE Ren-gao
Department of Life & Science,Qingdao Agricultural University,Qingdao 266109,Shandong,China
Keywords:
Soybean; Cotyledonary node fragment; Cotyledonary node slice; Callus; Plant regeneration
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0546
Abstract:
Effects of germination medium and different concentration ratios of BA and TDZ on the callus induction and plant regeneration were studied using fragments and slices of 7 day-germinated soybean cotyledonary nodes as explants.The result showed that the callus formation occurred from the cotyledonary node fragments only when it was cultured on germination medium containing BA.There were significant differences in callus induction frequencies among different explants,different concentration ratios of BA and TDZ on callus induction medium.The induction frequency of callus was low for cotyledonary node fragment explants(average less than 20%)under the combination concentration of BA( 0.05-0.15 mg?L-1)and TDZ(0.04-0.12 mg?L-1),the highest induction frequency (50%) was obtained when cotyledonary node fragments were cultured on callus induction medium containing 0.15 mg?L-1 BA and 0.08 mg?L-1 TDZ.However,the induction frequency of callus was significantly enhanced by using the cotyledonary node slices as explants(average more than 85%),the highest frequency reached 95%.The callus induced was transferred to differentiation medium containing BA for shoot induction.The highest induction frequency of shoots （21.4%）was obtained on differentiation medium containing 0.2 mg?L-1BA.

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