Construction of Expression Vector pCAAFP66 and Its Transformation into Soybean by Agrobacterium-mediated Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2011年04期

Page:

541-545

Research Field:

Publishing date:

Info

Title:

Construction of Expression Vector pCAAFP66 and Its Transformation into Soybean by Agrobacterium-mediated Method

Author(s):

ZHAO Yin-hua¹; CHEN Ying-shan¹; GUO Li-qiong¹; 2; QIN Feng-yun¹; LIN Jun-fang¹; 2

1.College of Food Science,South China Agricultural University,Guangzhou 510642；

?2.Institute of Biomass Research,South China Agricultural University,Guangzhou 510642,Guangdong,China

Keywords:

afp gene; Vector construction; Agrobacterium-mediated; Soybean genetic transformation

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2011.04.0541

Abstract:

Plant expression vector pCAAFP66(9868 bp),which contained the target gene afp and the screening marker Bar gene,was constructed for soybean transformation.The anti-freezing gene afp was transformed into embryonic tip and cotyledonary node of soybean using Agrobacterium-mediated method and PPT as selective agent.Identification indicated the afpgene was successfully transformed into Huachun 3 and Huachun 6 through the embryonic tip transformation system,with transformation rates 7.4% and 4.0%, respectively.Whereas in the cotyledonary node system no transformed plants was obtained.Analysis of the embryonic tip and cotyledonary node approach revealed that significant differences existed in the regeneration and transformation rate between two genotypes;even with the same genotype,different explants materials had totally different transformation rates.

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