|Table of Contents|

Preparation of Polyclonal Antibody against D-AtCGS and Detection of D-AtCGS in Transgenic Soybean Plants(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2011年04期
Page:
537-540
Research Field:
Publishing date:

Info

Title:
Preparation of Polyclonal Antibody against D-AtCGS and Detection of D-AtCGS in Transgenic Soybean Plants
Author(s):
WANG Yi-peng1;  3;  YU Yang2;  SUN Shi2;  XU Yan-li1;  HOU Wen-sheng2
1. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150040, Heilongjiang;
?2. National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081;
3.College of Life Science, Northeast Forestry University, Harbin 150040, Heilongjiang, China
Keywords:
D-AtCGS;  Prokaryotic expression;  Preparation of polyclonal antibody;  Detection of transgenic plants
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.04.0537
Abstract:
Methionine（Met） has very important physiological functions as an essential sulfur-containing-amino acid. Cystathionine-γ-synthase (CGS) is the key enzyme in Met synthesis. In this study, prokaryotic expression vector of Arabidopsis D-AtCGS gene was constructed, and D-AtCGS protein was purified to prepare polyclonal antibody against D-AtCGS. Then transgenic soybean constitutive expressing or seeds specific expressing D-AtCGS and Lotus corniculatus transgenic plants with F-AtCGS or D-AtCGS obtained by Beijing Branch Center of National Center for Soybean Improvement were detected by Western blot method using polyclonal antibody we prepared. Results showed that detection signal of transgenic plants were all much stronger than wild type. At last, Western blot method for detection of transgenic soybean plants with AtCGS gene was successfully established. This work provides technology methods for analysis of CGS expression and detection of CGS transgenic plants.

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Last Update: 2014-08-14